• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 32452-1-AP

    WB analysis using 32452-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32452-1-AP (BRD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 32452-1-AP

    IP experiment of Jurkat using 32452-1-AP

    IP result of anti-BRD1/BRL (IP:32452-1-AP, 4ug; Detection:32452-1-AP 1:1000) with Jurkat cells lysate 1920 ug.

BRD1/BRL Polyclonal antibody

BRD1/BRL Polyclonal Antibody for WB, IP, ELISA
Cat No. 32452-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

BR140-like protein, BRD1, BRL, Bromodomain and PHD finger-containing protein 2, Bromodomain-containing protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inJurkat cells, SH-SY5Y cells, SK-N-SH cells
Positive IP detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32452-1-AP targets BRD1/BRL in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原 BRD1/BRL fusion protein Ag37530 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bromodomain containing 1
别名 BR140-like protein, BRD1, BRL, Bromodomain and PHD finger-containing protein 2, Bromodomain-containing protein 1
计算分子量 120 kDa
观测分子量133 kDa
GenBank蛋白编号BC047508
基因名称 BRD1
Gene ID (NCBI) 23774
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO95696
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BRD1, also known as BRL and BRPF2, plays a key role in chromatin recruitment to modulate histone acetylation and consequently gene transcription. It interacts with a range of epigenetic modifiers, including histone acetyltransferases KAT5 and KAT7, as well as the histone-lysine N-methyltransferase KMT5B (PMID: 29453316, 38383216). It has 2 isoforms with the molecular mass of 133 kDa and 120 kDa.

实验方案

Product Specific Protocols
WB protocol for BRD1/BRL antibody 32452-1-APDownload protocol
IP protocol for BRD1/BRL antibody 32452-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols