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验证数据展示

  • WB analysis using 85430-4-RR

    WB analysis using 85430-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85430-4-RR (BRCC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 85430-4-RR

    WB analysis using 85430-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85430-4-RR (BRCC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of SK-BR-3 using 85430-4-RR

    IP experiment of SK-BR-3 using 85430-4-RR

    IP result of anti-BRCC3 (IP:85430-4-RR, 4ug; Detection:85430-4-RR 1:2000) with SK-BR-3 cells lysate 1280 ug.

  • IHC staining of human ovary cancer using 85430-4-RR

    IHC staining of human ovary cancer using 85430-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85430-4-RR (BRCC3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BRCC3 antibody included
  • IHC staining of Human placenta using 85430-4-RR

    IHC staining of Human placenta using 85430-4-RR

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue slide using 85430-4-RR (BRCC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BRCC3 antibody included
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BRCC3 Recombinant monoclonal antibody

BRCC3 Uni-rAb® Recombinant Antibody for WB, IHC, IP, ELISA
Cat No. 85430-4-RR

产品说明书

CloneNo. 242931B9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

BRCA1/BRCA2-containing complex subunit 3, BRCA1/BRCA2-containing complex subunit 36, BRCA1-A complex subunit BRCC36, BRCC36, BRISC complex subunit BRCC36

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSK-BR-3 cells, HeLa cells, HEK-293T cells, MCF-7 cells, HT-29 cells, Neuro-2a cells, HSC-T6 cells
Positive IP detected inSK-BR-3 cells
Positive IHC detected inhuman ovary cancer tissue, Human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy BRCC3 Ready-To-Use IHC Kit. BRCC3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85430-4-RR targets BRCC3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7584

Product name: Recombinant human BRCC3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 14-316 aa of BC002999

Sequence: LESDAFLVCLNHALSTEKEEVMGLCIGELNDDTRSDSKFAYTGTEMRTVAEKVDAVRIVHIHSVIILRRSDKRKDRVEISPEQLSAASTEAERLAELTGRPMRVVGWYHSHPHITVWPSHVDVRTQAMYQMMDQGFVGLIFSCFIEDKNTKTGRVLYTCFQSIQAQKSSESLHGPRDFWSSSQHISIEGQKEEERYERIEIPIHIVPHVTIGKVCLESAVELPKILCQEEQDAYRRIHSLTHLDSVTKIHNGSVFTKNLCSQMSAVSGPLLQWLEDRLEQNQQHLQELQQEKEELMQELSSLE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 BRCA1/BRCA2-containing complex, subunit 3
别名 BRCA1/BRCA2-containing complex subunit 3, BRCA1/BRCA2-containing complex subunit 36, BRCA1-A complex subunit BRCC36, BRCC36, BRISC complex subunit BRCC36
计算分子量 36 kDa
观测分子量 35 kDa
GenBank蛋白编号BC002999
基因名称 BRCC3
Gene ID (NCBI) 79184
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP46736
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BRCA1-BRCA2-containing complex subunit 3 (BRCC3), a Lys-63-specific deubiquitinase, is a member of the JAMM/MPN family of zinc metalloproteases. BRCC3 have been shown to promote the inflammasome activation by deubiquitinating NOD-like receptor containing pyrin domain 3 (NLRP3). BRCC3 is associated with G2/M arrest in breast cancer cells and DNA damage. The expression of BRCC3 is associated with increased cell proliferation. (PMID: 23246432, PMID: 30272359)

实验方案

Product Specific Protocols
WB protocol for BRCC3 antibody 85430-4-RRDownload protocol
IHC protocol for BRCC3 antibody 85430-4-RRDownload protocol
IP protocol for BRCC3 antibody 85430-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols