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验证数据展示

  • WB analysis using 85430-4-RR (same clone as 85430-4-PBS)

    WB analysis using 85430-4-RR (same clone as 85430-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85430-4-RR (BRCC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85430-4-PBS in a different storage buffer formulation.

  • WB analysis using 85430-4-RR (same clone as 85430-4-PBS)

    WB analysis using 85430-4-RR (same clone as 85430-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85430-4-RR (BRCC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85430-4-PBS in a different storage buffer formulation.

  • IP experiment of SK-BR-3 using 85430-4-RR (same clone as 85430-4-PBS)

    IP experiment of SK-BR-3 using 85430-4-RR (same clone as 85430-4-PBS)

    IP result of anti-BRCC3 (IP:85430-4-RR, 4ug; Detection:85430-4-RR 1:2000) with SK-BR-3 cells lysate 1280 ug. This data was developed using the same antibody clone with 85430-4-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 85430-4-RR (same clone as 85430-4-PBS)

    IHC staining of human ovary cancer using 85430-4-RR (same clone as 85430-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85430-4-RR (BRCC3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85430-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BRCC3 antibody included
  • IHC staining of Human placenta using 85430-4-RR (same clone as 85430-4-PBS)

    IHC staining of Human placenta using 85430-4-RR (same clone as 85430-4-PBS)

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue slide using 85430-4-RR (BRCC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85430-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BRCC3 antibody included

BRCC3 Recombinant monoclonal antibody, PBS Only

BRCC3 Uni-rAb® Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 85430-4-PBS

产品说明书

CloneNo. 242931B9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

BRCA1/BRCA2-containing complex subunit 3, BRCA1/BRCA2-containing complex subunit 36, BRCA1-A complex subunit BRCC36, BRCC36, BRISC complex subunit BRCC36

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy BRCC3 Ready-To-Use IHC Kit. BRCC3 primary antibody included.

产品信息

85430-4-PBS targets BRCC3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7584

Product name: Recombinant human BRCC3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 14-316 aa of BC002999

Sequence: LESDAFLVCLNHALSTEKEEVMGLCIGELNDDTRSDSKFAYTGTEMRTVAEKVDAVRIVHIHSVIILRRSDKRKDRVEISPEQLSAASTEAERLAELTGRPMRVVGWYHSHPHITVWPSHVDVRTQAMYQMMDQGFVGLIFSCFIEDKNTKTGRVLYTCFQSIQAQKSSESLHGPRDFWSSSQHISIEGQKEEERYERIEIPIHIVPHVTIGKVCLESAVELPKILCQEEQDAYRRIHSLTHLDSVTKIHNGSVFTKNLCSQMSAVSGPLLQWLEDRLEQNQQHLQELQQEKEELMQELSSLE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 BRCA1/BRCA2-containing complex, subunit 3
别名 BRCA1/BRCA2-containing complex subunit 3, BRCA1/BRCA2-containing complex subunit 36, BRCA1-A complex subunit BRCC36, BRCC36, BRISC complex subunit BRCC36
计算分子量 36 kDa
观测分子量 35 kDa
GenBank蛋白编号BC002999
基因名称 BRCC3
Gene ID (NCBI) 79184
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP46736
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BRCA1-BRCA2-containing complex subunit 3 (BRCC3), a Lys-63-specific deubiquitinase, is a member of the JAMM/MPN family of zinc metalloproteases. BRCC3 have been shown to promote the inflammasome activation by deubiquitinating NOD-like receptor containing pyrin domain 3 (NLRP3). BRCC3 is associated with G2/M arrest in breast cancer cells and DNA damage. The expression of BRCC3 is associated with increased cell proliferation. (PMID: 23246432, PMID: 30272359)

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