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验证数据展示

  • WB analysis of human kidney using 17974-1-AP

    WB analysis of human kidney using 17974-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 17974-1-AP (BPHL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 17974-1-AP

    IHC staining of human kidney using 17974-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 17974-1-AP

    IHC staining of human kidney using 17974-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 40x lens).

BPHL Polyclonal antibody

BPHL Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17974-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

VACVASE, Poly(ADP-ribose) glycohydrolase, PARG, MCNAA, EC:3.2.1.143

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman kidney tissue, human liver tissue, MCF-7 cells, mouse kidney tissue
Positive IHC detected inhuman kidney tissue, human heart tissue, human lung tissue, human ovary tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17974-1-AP targets BPHL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 BPHL fusion protein Ag12085 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 biphenyl hydrolase-like (serine hydrolase)
别名 VACVASE, Poly(ADP-ribose) glycohydrolase, PARG, MCNAA, EC:3.2.1.143
计算分子量 22 kDa, 32 kDa
观测分子量 27 kDa
GenBank蛋白编号BC037778
基因名称 BPHL
Gene ID (NCBI) 670
RRIDAB_2274994
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86WA6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BPHL antibody 17974-1-APDownload protocol
IHC protocol for BPHL antibody 17974-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols