验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, RT-4 cells, U-251 cells, U-87 MG cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33196-1-AP targets BORC6 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag37224 Product name: Recombinant human C17orf59 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-223 aa of BC018880 Sequence: MDVEQQEEEDNDEEAAAGSRAGRSFSSRLQDSRSLDGLSEACGGAGSSGSAESGAGGGRRATISSPLELEGTVSRHGDLTHFVANNLQLKIRLSGAPPPPPSAPARPCPAPAPTPTPAIPPIDPEVLRDLERLSRELGGRVDRLLRGLGGAVQELTALSVGCIQTYRDAVDSLGEAVDMSIKGMYTLLARCEELERALQPVQGLARQVRDIRRTLEVLEALCK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 17 open reading frame 59 |
| 别名 | BLOC-1-related complex subunit 6, BORCS6, C17orf59, Lysosome-dispersing protein, Lyspersin |
| 计算分子量 | 357 aa, 37 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC018880 |
| 基因名称 | C17orf59 |
| Gene ID (NCBI) | 54785 |
| RRID | AB_3742890 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q96GS4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BORC6 is a part of the BORC complex, which may play a role in lysosomes movement and localization at the cell periphery. Associated with the cytosolic face of lysosomes, the BORC complex may recruit ARL8B and couple lysosomes to microtubule plus-end-directed kinesin motor (PMID: 25898167).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BORC6 antibody 33196-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
