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验证数据展示

  • WB analysis using 26696-1-AP

    WB analysis using 26696-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26696-1-AP (BMPR1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 26696-1-AP

    IF Staining of MCF-7 using 26696-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using BMPR1B antibody (26696-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

BMPR1B Polyclonal antibody

BMPR1B Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26696-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

ALK 6, ALK6, BMP type 1B receptor, BMPR 1B, BMPR1B, CDw293

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse colon tissue, rat colon tissue
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

26696-1-AP targets BMPR1B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 BMPR1B fusion protein Ag24902 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bone morphogenetic protein receptor, type IB
别名 ALK 6, ALK6, BMP type 1B receptor, BMPR 1B, BMPR1B, CDw293
计算分子量 57 kDa
观测分子量 50 kDa
GenBank蛋白编号BC047773
基因名称 BMPR1B
Gene ID (NCBI) 658
RRIDAB_3085892
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00238
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BMPR1B (Bone morphogenetic protein receptor type-1B) is also named as CDw293. BMPR1B belongs to the protein kinase superfamily. BMPR1B is on ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. BMPR1B is receptor for BMP7/OP-1 and GDF5. BMPR1B Positively regulates chondrocyte differentiation through GDF5 interaction.

实验方案

Product Specific Protocols
WB protocol for BMPR1B antibody 26696-1-APDownload protocol
IF protocol for BMPR1B antibody 26696-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Neurooncol

The effects of BMP2 and the mechanisms involved in the invasion and angiogenesis of IDH1 mutant glioma cells

Authors - Hui Xu
View Article