验证数据展示
经过测试的应用
| Positive WB detected in | Recombinant protein |
| Positive IHC detected in | human liver cancer tissue, human lung cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
22188-1-AP targets BMP3 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag16750 Product name: Recombinant human BMP3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 22-349 aa of BC096269 Sequence: GERPKPPFPELRKAVPGDRTAGGGPDSELQPQDKVSEHMLRLYDRYSTVQAARTPGSLEGGSQPWRPRLLREGNTVRSFRAAAAETLERKGLYIFNLTSLTKSENILSATLYFCIGELGNISLSCPVSGGCSHHAQRKHIQIDLSAWTLKFSRNQSQLLGHLSVDMAKSHRDIMSWLSKDITQFLRKAKENEEFLIGFNITSKGRQLPKRRLPFPEPYILVYANDAAISEPESVVSSLQGHRNFPTGTVPKWDSHIRAALSIERRKKRSTGVLLPLQNNELPGAEYQYKKDEVWEERKPYKTLQAQAPEKSKNKKKQRKGPHRKSQTL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | bone morphogenetic protein 3 |
| 别名 | BMP 3, BMP 3A, BMP3, BMP-3, BMP3A, bone morphogenetic protein 3, Bone morphogenetic protein 3A, Osteogenin |
| 计算分子量 | 472 aa, 53 kDa |
| GenBank蛋白编号 | BC096269 |
| 基因名称 | BMP3 |
| Gene ID (NCBI) | 651 |
| RRID | AB_2879019 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P12645 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BMP3 belongs to the transforming growth factor-beta (TGFB) superfamily. BMP3 plays an important role in embryonic development by inducing and patterning early skeletal formation. BMP3 has also been reported as a negative growth regulator in bone marrow progenitor cells through the inhibition of DNA synthesis and proliferation. Additionally, recent studies have revealed that BMP3 contributes to tumor development and progression.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for BMP3 antibody 22188-1-AP | Download protocol |
| WB protocol for BMP3 antibody 22188-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biochem Biophys Res Commun Bone morphogenetic protein-3 is a negative regulator of transforming growth factor beta and fibrosis | ||
Biol Trace Elem Res Effects of Sodium Fluoride Treatment In Vitro on Cell Proliferation, BMP-2 and BMP-3 Expression in Human Osteosarcoma MG-63 Cells. |
