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验证数据展示

  • WB analysis of Recombinant protein using 22188-1-AP

    WB analysis of Recombinant protein using 22188-1-AP

    Recombinant protein were subjected to SDS PAGE followed by western blot with 22188-1-AP (BMP3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 22188-1-AP

    IHC staining of human liver cancer using 22188-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22188-1-AP (BMP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 22188-1-AP

    IHC staining of human liver cancer using 22188-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22188-1-AP (BMP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

BMP3 Polyclonal antibody

BMP3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22188-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

BMP 3, BMP 3A, BMP3, BMP-3, BMP3A, bone morphogenetic protein 3, Bone morphogenetic protein 3A, Osteogenin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRecombinant protein
Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

22188-1-AP targets BMP3 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 BMP3 fusion protein Ag16750 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bone morphogenetic protein 3
别名 BMP 3, BMP 3A, BMP3, BMP-3, BMP3A, bone morphogenetic protein 3, Bone morphogenetic protein 3A, Osteogenin
计算分子量 472 aa, 53 kDa
GenBank蛋白编号BC096269
基因名称 BMP3
Gene ID (NCBI) 651
RRIDAB_2879019
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP12645
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BMP3 belongs to the transforming growth factor-beta (TGFB) superfamily. BMP3 plays an important role in embryonic development by inducing and patterning early skeletal formation. BMP3 has also been reported as a negative growth regulator in bone marrow progenitor cells through the inhibition of DNA synthesis and proliferation. Additionally, recent studies have revealed that BMP3 contributes to tumor development and progression.

实验方案

Product Specific Protocols
WB protocol for BMP3 antibody 22188-1-APDownload protocol
IHC protocol for BMP3 antibody 22188-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

Bone morphogenetic protein-3 is a negative regulator of transforming growth factor beta and fibrosis

Authors - Emma R Dorris
View Article
humanWB

Biol Trace Elem Res

Effects of Sodium Fluoride Treatment In Vitro on Cell Proliferation, BMP-2 and BMP-3 Expression in Human Osteosarcoma MG-63 Cells.

Authors - Yan Wei
View Article