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验证数据展示

  • WB analysis of pig bone marrow using 66383-1-Ig (same clone as 66383-1-PBS)

    WB analysis of pig bone marrow using 66383-1-Ig (same clone as 66383-1-PBS)

    pig bone marrow tissue were subjected to SDS PAGE followed by western blot with 66383-1-Ig (BMP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66383-1-PBS in a different storage buffer formulation.

  • IHC staining of human small intestine using 66383-1-Ig (same clone as 66383-1-PBS)

    IHC staining of human small intestine using 66383-1-Ig (same clone as 66383-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66383-1-Ig (BMP2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66383-1-PBS in a different storage buffer formulation.

  • WB analysis using 66383-1-Ig (same clone as 66383-1-PBS)

    WB analysis using 66383-1-Ig (same clone as 66383-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66383-1-Ig (BMP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66383-1-PBS in a different storage buffer formulation.

  • IHC staining of human small intestine using 66383-1-Ig (same clone as 66383-1-PBS)

    IHC staining of human small intestine using 66383-1-Ig (same clone as 66383-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66383-1-Ig (BMP2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66383-1-PBS in a different storage buffer formulation.

BMP2 Monoclonal antibody, PBS Only

BMP2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66383-1-PBS

产品说明书

CloneNo. 1C5C2

宿主/亚型

Mouse / IgG2a

种属反应性

human, pig

应用

WB, IHC, Indirect ELISA

别名

BMP 2, BMP2A, BMP-2, BMP-2A, BMP 2A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66383-1-PBS targets BMP2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, pig
免疫原

CatNo: Ag13501

Product name: Recombinant human BMP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-396 aa of BC069214

Sequence: MVAGTRCLLALLLPQVLLGGAAGLVPELGRRKFAAASSGRPSSQPSDEVLSEFELRLLSMFGLKQRPTPSRDAVVPPYMLDLYRRHSGQPGSPAPDHRLERAASRANTVRSFHHEESLEELPETSGKTTRRFFFNLSSIPTEEFITSAELQVFREQMQDALGNNSSFHHRINIYEIIKPATANSKFPVTSLLDTRLVNQNASRWESFDVTPAVMRWTAQGHANHGFVVEVAHLEEKQGVSKRHVRISRSLHQDEHSWSQIRPLLVTFGHDGKGHPLHKREKRQAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 bone morphogenetic protein 2
别名 BMP 2, BMP2A, BMP-2, BMP-2A, BMP 2A
计算分子量 396 aa, 44 kDa
观测分子量 45 kDa
GenBank蛋白编号BC069214
基因名称 BMP2
Gene ID (NCBI) 650
RRIDAB_2881759
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP12643
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor-beta (TGFB) superfamily. BMP2 is synthesized as a 60 kDa precursor that is processed in the secretory pathway to a small 18 kDa monomer; 2 monomers then associate to form the active 30 kDa homodimer, which binds to its receptor. There is also a 40-45 kDa form of BMP2, as an amino-terminal propeptide. BMP2 can induce bone formation and regeneration during early embryonic development. It is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction. Two band isofroms of BMP2 are present (PMID: 35846837).