验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18982-1-AP targets BMP15 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | bone morphogenetic protein 15 |
| 别名 | BMP 15, BMP15, BMP-15, bone morphogenetic protein 15, GDF 9B, GDF9B, ODG2, POF4 |
| 计算分子量 | 45 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | NM_005448 |
| 基因名称 | BMP15 |
| Gene ID (NCBI) | 9210 |
| RRID | AB_2878570 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95972 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BMP15, also named as GDF9B, is a member of the bone morphogenetic protein family which is part of the transforming growth factor beta superfamily. The transforming growth factor beta superfamily includes large families of growth and differentiation factors. BMP15 may be involved follicular development and oocyte-specific growth/differentiation factor that stimulates folliculogenesis and granulosa cell (GC) growth. Mutation of BMP15 will cause of ovarian dysgenesis type 2 (ODG2) and premature ovarian failure type 4 (POF4).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BMP15 antibody 18982-1-AP | Download protocol |
| IHC protocol for BMP15 antibody 18982-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Commun Signal GDF9His209GlnfsTer6/S428T and GDF9Q321X/S428T bi-allelic variants caused female subfertility with defective follicle enlargement | ||
Mol Med Rep Abnormal expression levels of BMP15/Smad1 are associated with granulosa cell apoptosis in patients with polycystic ovary syndrome. | ||
Proc Natl Acad Sci U S A A rare germline BMP15 missense mutation causes hereditary ovarian immature teratoma in human | ||
Adv Sci (Weinh) AMPK Suppression Due to Obesity Drives Oocyte mtDNA Heteroplasmy via ATF5-POLG Axis | ||
Environ Toxicol Acrylamide Exposure Impairs Ovarian Tricarboxylic Acid Cycle and Reduces Oocyte Quality in Mouse | ||
Reprod Sci Melatonin Protects Against Mitochondrial Dyshomeostasis and Ovarian Damage Caused by Chronic Unpredictable Mild Stress Through the eIF2α-AFT4 Signaling Pathway in Mice |
