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验证数据展示

  • WB analysis using 87836-1-RR (same clone as 87836-1-PBS)

    WB analysis using 87836-1-RR (same clone as 87836-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87836-1-RR (BMI1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87836-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 87836-1-RR (same clone as 87836-1-PBS)

    WB analysis of HEK-293 using 87836-1-RR (same clone as 87836-1-PBS)

    WB result of BMI1 antibody (87836-1-RR; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BMI1 transfected HEK-293 cells. This data was developed using the same antibody clone with 87836-1-PBS in a different storage buffer formulation.

  • Affinity of 87836-1-RR

    Affinity of 87836-1-RR

    Biolayer interferometry (BLI) kinetic assays of 87836-1-RR against Human BMI1 were performed. The affinity constant is below 1 pM.

BMI1 Recombinant monoclonal antibody, PBS Only

BMI1 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 87836-1-PBS

产品说明书

CloneNo. 253339C8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

PCGF4, Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RNF51

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy BMI1 Ready-To-Use IHC Kit. BMI1 primary antibody included.

产品信息

87836-1-PBS targets BMI1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1286

Product name: Recombinant human BMI1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-326 aa of BC011652

Sequence: MHRTTRIKITELNPHLMCVLCGGYFIDATTIIECLHSFCKTCIVRYLETSKYCPICDVQVHKTRPLLNIRSDKTLQDIVYKLVPGLFKNEMKRRRDFYAAHPSADAANGSNEDRGEVADEDKRIITDDEIISLSIEFFDQNRLDRKVNKDKEKSKEEVNDKRYLRCPAAMTVMHLRKFLRSKMDIPNTFQIDVMYEEEPLKDYYTLMDIAYIYTWRRNGPLPLKYRVRPTCKRMKISHQRDGLTNAGELESDSGSDKANSPAGGIPSTSSCLPSPSTPVQSPHPQFPHISSTMNGTSNSPSGNHQSSFANRPRKSSVNGSSATSSG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 BMI1 polycomb ring finger oncogene
别名 PCGF4, Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RNF51
计算分子量 37 kDa
观测分子量41-43 kDa
GenBank蛋白编号BC011652
基因名称 BMI1
Gene ID (NCBI) 648
RRIDAB_3745777
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP35226
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BMI- 1 is one of polycomb group genes, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16Ink4a and p19Arf genes and is required for maintenance of adult hematopoietic and neural stem cells. The antibody 10832-1-AP detected proteins of 45-48kda, which may include phosphorylated isoforms of the protein.

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