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验证数据展示

  • WB analysis of PC-12 using 66161-1-Ig (same clone as 66161-1-PBS)

    WB analysis of PC-12 using 66161-1-Ig (same clone as 66161-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 66161-1-Ig (BMI1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 66161-1-Ig (same clone as 66161-1-PBS)

    IP experiment of HEK-293 using 66161-1-Ig (same clone as 66161-1-PBS)

    IP result of anti-BMI1 (IP:66161-1-Ig, 3ug; Detection:66161-1-Ig 1:1000) with HEK-293 cells lysate 1800ug. This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 66161-1-Ig (same clone as 66161-1-PBS)

    IHC staining of human lymphoma using 66161-1-Ig (same clone as 66161-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66161-1-Ig (BMI1 Antibody) at dilution of 1:50 under 40x lens. This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BMI1 antibody included
  • IHC staining of human breast cancer using 66161-1-Ig (same clone as 66161-1-PBS)

    IHC staining of human breast cancer using 66161-1-Ig (same clone as 66161-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66161-1-Ig (BMI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BMI1 antibody included
  • IF Staining of HEK-293 using 66161-1-Ig (same clone as 66161-1-PBS)

    IF Staining of HEK-293 using 66161-1-Ig (same clone as 66161-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using BMI1 antibody (66161-1-Ig, Clone: 2E10A11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66161-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51443-1

    Cytometric bead array sample test of MP51443-1

    Cytometric bead array sample test of MP51443-1, BMI1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66161-2-PBS. Detection antibody: 66161-1-PBS.

  • Cytometric bead array standard curve of MP51443-1

    Cytometric bead array standard curve of MP51443-1

    Cytometric bead array standard curve of MP51443-1, BMI1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66161-2-PBS. Detection antibody: 66161-1-PBS. Standard:Ag21284. Range: 0.391-100 ng/mL.

  • Featured Product
  • KD/KO验证

BMI1 Monoclonal antibody, PBS Only (Detector)

BMI1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA
Cat No. 66161-1-PBS

产品说明书

CloneNo. 2E10A11

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, monkey

应用

WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

别名

2E10A11, PCGF4, Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RNF51

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy BMI1 Ready-To-Use IHC Kit. BMI1 primary antibody included.

产品信息

66161-1-PBS targets BMI1 as part of a matched antibody pair:

MP51443-1: 66161-2-PBS capture and 66161-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, monkey
免疫原

CatNo: Ag21284

Product name: Recombinant human BMI1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-326 aa of BC011652

Sequence: MHRTTRIKITELNPHLMCVLCGGYFIDATTIIECLHSFCKTCIVRYLETSKYCPICDVQVHKTRPLLNIRSDKTLQDIVYKLVPGLFKNEMKRRRDFYAAHPSADAANGSNEDRGEVADEDKRIITDDEIISLSIEFFDQNRLDRKVNKDKEKSKEEVNDKRYLRCPAAMTVMHLRKFLRSKMDIPNTFQIDVMYEEEPLKDYYTLMDIAYIYTWRRNGPLPLKYRVRPTCKRMKISHQRDGLTNAGELESDSGSDKANSPAGGIPSTSSCLPSPSTPVQSPHPQFPHISSTMNGTSNSPSGNHQSSFANRPRKSSVNGSSATSSG

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 BMI1 polycomb ring finger oncogene
别名 2E10A11, PCGF4, Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RNF51
计算分子量 37 kDa
观测分子量37-43 kDa
GenBank蛋白编号BC011652
基因名称 BMI1
Gene ID (NCBI) 648
RRIDAB_2881557
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP35226
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BMI- 1 is one of polycomb group genes, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16Ink4a and p19Arf genes and is required for maintenance of adult hematopoietic and neural stem cells. The antibody reacts with the 37kd BMI1 protein.