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验证数据展示

  • WB analysis of Jurkat using 10510-1-AP (same clone as 10510-1-PBS)

    WB analysis of Jurkat using 10510-1-AP (same clone as 10510-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10510-1-AP (BLK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10510-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 10510-1-AP (same clone as 10510-1-PBS)

    IHC staining of human pancreas cancer using 10510-1-AP (same clone as 10510-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10510-1-AP (BLK Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10510-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 10510-1-AP (same clone as 10510-1-PBS)

    IHC staining of human pancreas cancer using 10510-1-AP (same clone as 10510-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10510-1-AP (BLK Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10510-1-PBS in a different storage buffer formulation.

  • IP experiment of SH-SY5Y using 10510-1-AP (same clone as 10510-1-PBS)

    IP experiment of SH-SY5Y using 10510-1-AP (same clone as 10510-1-PBS)

    IP result of anti-BLK (IP:10510-1-AP, 4ug; Detection:10510-1-AP 1:500) with SH-SY5Y cells lysate 1600ug. This data was developed using the same antibody clone with 10510-1-PBS in a different storage buffer formulation.

BLK Polyclonal antibody, PBS Only

BLK Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 10510-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

10510-1-PBS targets BLK in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0795

Product name: Recombinant human BLK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-100 aa of BC007371

Sequence: MGLVSSKKPDKEKPIKEKDKGQWSPLKVSAQDKDAPPLPPLVVFNHLTPPPPDEHLDEDKHFVVALYDYTAMNDRDLQMLKGEKLQVLKGTGDWWLARSL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 B lymphoid tyrosine kinase
别名
计算分子量 58 kDa
观测分子量 58 kDa
GenBank蛋白编号BC007371
基因名称 BLK
Gene ID (NCBI) 640
RRIDAB_2274754
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51451
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tyrosine-protein kinase Blk(BLK) is typically involved in cell proliferation and differentiation.BLK is a 58 kDa protein with important role in B-cell receptor signaling and B-cell development. BLK also stimulates INS synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors.

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