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BLK Polyclonal antibody

BLK Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 10510-1-AP

Print datasheet

Synonyms

B lymphocyte kinase, B lymphoid tyrosine kinase, BLK, p55 Blk, Tyrosine protein kinase Blk

验证数据展示

  • WB analysis of Jurkat using 10510-1-AP

    WB analysis of Jurkat using 10510-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10510-1-AP (BLK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 10510-1-AP

    IHC staining of human pancreas cancer using 10510-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10510-1-AP (BLK Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 10510-1-AP

    IHC staining of human pancreas cancer using 10510-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10510-1-AP (BLK Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of SH-SY5Y using 10510-1-AP

    IP experiment of SH-SY5Y using 10510-1-AP

    IP result of anti-BLK (IP:10510-1-AP, 4ug; Detection:10510-1-AP 1:500) with SH-SY5Y cells lysate 1600ug.

经过测试的应用

Positive WB detected inJurkat cells, Raji cells, SH-SY5Y cells
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10510-1-AP targets BLK in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB,ELISA
Cited ApplicationsWB, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen BLK fusion protein Ag0795 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name B lymphoid tyrosine kinase
Synonyms B lymphocyte kinase, B lymphoid tyrosine kinase, BLK, p55 Blk, Tyrosine protein kinase Blk
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC007371
Gene Symbol BLK
Gene ID (NCBI) 640
RRIDAB_2274754
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51451
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tyrosine-protein kinase Blk(BLK) is typically involved in cell proliferation and differentiation.BLK is a 58 kDa protein with important role in B-cell receptor signaling and B-cell development. BLK also stimulates INS synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors.

实验方案

Product Specific Protocols
WB protocol for BLK antibody 10510-1-APDownload protocol
IHC protocol for BLK antibody 10510-1-APDownload protocol
IP protocol for BLK antibody 10510-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Signal Transduct Target Ther

B-lymphoid tyrosine kinase-mediated FAM83A phosphorylation elevates pancreatic tumorigenesis through interacting with β-catenin

Authors - Cefan Zhou
View Article