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验证数据展示

  • WB analysis using 29760-1-AP (same clone as 29760-1-PBS)

    WB analysis using 29760-1-AP (same clone as 29760-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29760-1-AP (BIRC6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29760-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 29760-1-AP (same clone as 29760-1-PBS)

    IHC staining of human breast cancer using 29760-1-AP (same clone as 29760-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29760-1-AP (BIRC6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29760-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BIRC6 antibody included
  • IHC staining of human breast cancer using 29760-1-AP (same clone as 29760-1-PBS)

    IHC staining of human breast cancer using 29760-1-AP (same clone as 29760-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29760-1-AP (BIRC6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29760-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BIRC6 antibody included
  • IF Staining of U2OS using 29760-1-AP (same clone as 29760-1-PBS)

    IF Staining of U2OS using 29760-1-AP (same clone as 29760-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using BIRC6 antibody (29760-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29760-1-PBS in a different storage buffer formulation.

BIRC6 Polyclonal antibody, PBS Only

BIRC6 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29760-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy BIRC6 Ready-To-Use IHC Kit. BIRC6 primary antibody included.

产品信息

29760-1-PBS targets BIRC6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30729

Product name: Recombinant human BIRC6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 4710-4857 aa of NM_016252

Sequence: GYERSRGTPSGTQSSREYDGNIRQATVKWAMLEQIRNPSPCFKEVIHKHFYLKRVEIMAQCEEWIADIQQYSSDKRVGRTMSHHAAALKRHTAQLREELLKLPCPEGLDPDTDDAPEVCRATTGAEETLMHDQVKPSSSKELPSDFQL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 baculoviral IAP repeat-containing 6
别名
计算分子量 530 kDa
观测分子量530 kDa
GenBank蛋白编号NM_016252
基因名称 BIRC6
Gene ID (NCBI) 57448
RRIDAB_2923604
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NR09
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BIRC6 (Baculoviral IAP Repeat Containing 6), also known as BRUCE or APOLLON, is a member of the Inhibitors of Apoptosis Protein (IAP) family with a single BIR domain at its N-terminal and an active ubiquitin-conjugating (UBC) enzyme domain at its C-terminal(PMID: 23409057). In addition to its IAP activity, BIRC6 has the distinctive property of activity as a chimeric E2/E3 ubiquitin ligase in mammals. With its UBC domain, BIRC6 promotes the degradation of Smac and inhibits the activity of caspase-9, which play key roles in the initiation of apoptosis(PMID: 25933218). BIRC6 is highly expressed in many cancer types, where elevated expression of BIRC6 is significantly correlated with poor clinical outcome(PMID: 34729249).

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