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BIRC6 Polyclonal antibody

BIRC6 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29760-1-AP

Print datasheet

Synonyms

APOLLON, BIRC6, BRUCE, FLJ13726, FLJ13786, KIAA1289

验证数据展示

  • WB analysis using 29760-1-AP

    WB analysis using 29760-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29760-1-AP (BIRC6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 29760-1-AP

    IHC staining of human breast cancer using 29760-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29760-1-AP (BIRC6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 29760-1-AP

    IHC staining of human breast cancer using 29760-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29760-1-AP (BIRC6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 29760-1-AP

    IF Staining of U2OS using 29760-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using BIRC6 antibody (29760-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inDU 145 cells, LNCaP cells, PC-3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29760-1-AP targets BIRC6 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity Human
Cited Reactivityhuman, mouse
Immunogen BIRC6 fusion protein Ag30729 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name baculoviral IAP repeat-containing 6
Synonyms APOLLON, BIRC6, BRUCE, FLJ13726, FLJ13786, KIAA1289
Calculated Molecular Weight 530 kDa
Observed Molecular Weight530 kDa
GenBank Accession NumberNM_016252
Gene Symbol BIRC6
Gene ID (NCBI) 57448
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NR09
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BIRC6 (Baculoviral IAP Repeat Containing 6), also known as BRUCE or APOLLON, is a member of the Inhibitors of Apoptosis Protein (IAP) family with a single BIR domain at its N-terminal and an active ubiquitin-conjugating (UBC) enzyme domain at its C-terminal(PMID: 23409057). In addition to its IAP activity, BIRC6 has the distinctive property of activity as a chimeric E2/E3 ubiquitin ligase in mammals. With its UBC domain, BIRC6 promotes the degradation of Smac and inhibits the activity of caspase-9, which play key roles in the initiation of apoptosis(PMID: 25933218). BIRC6 is highly expressed in many cancer types, where elevated expression of BIRC6 is significantly correlated with poor clinical outcome(PMID: 34729249).

实验方案

Product Specific Protocols
WB protocol for BIRC6 antibody 29760-1-APDownload protocol
IHC protocol for BIRC6 antibody 29760-1-APDownload protocol
IF protocol for BIRC6 antibody 29760-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanWB,IHC

Front Genet

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Authors - Fang Tong
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