验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, A375 cells, HEK-293 cells, HeLa cells, mouse testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20186-1-AP targets BIN3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag14091 Product name: Recombinant human BIN3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-199 aa of BC001223 Sequence: MSKSAVKISLDLLSNPLCEQDQDLLNMVTALDTAMKRMDAFNQEKVNQIQKTVIEPLKKFGSVFPSLNMAVKRREQALQDYRRLQAKVEKYEEKEKTGPVLAKLHQAREELRPVREDFEAKNRQLLEEMPRFYGSRLDYFQPSFESLIRAQVVYYSEMHKIFGDLSHQLDQPGHSDEQRERENEAKLSELRALSIVADD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | bridging integrator 3 |
| 别名 | BIN3, bridging integrator 3 |
| 计算分子量 | 253 aa, 30 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC001223 |
| 基因名称 | BIN3 |
| Gene ID (NCBI) | 55909 |
| RRID | AB_10638782 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NQY0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BIN3, also named as MEPCE and BCDIN3, belongs to the methyltransferase superfamily. It is an S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to stabilize it.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BIN3 antibody 20186-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
