BHLHE41 Polyclonal antibody

BHLHE41 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12688-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (3)

hDEC2, Enhancer-of-split and hairy-related protein 1, DEC2, Class E basic helix-loop-helix protein 41, Class B basic helix-loop-helix protein 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman ovary tumor tissue, mouse brain tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy BHLHE41 Ready-To-Use IHC Kit. BHLHE41 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12688-1-AP targets BHLHE41 in WB, IHC, IF/ICC, IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF, IP, chIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 BHLHE41 fusion protein Ag3381 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 basic helix-loop-helix family, member e41
别名 hDEC2, Enhancer-of-split and hairy-related protein 1, DEC2, Class E basic helix-loop-helix protein 41, Class B basic helix-loop-helix protein 3
计算分子量 482 aa, 51 kDa
观测分子量 48-50 kDa
GenBank蛋白编号BC025968
基因名称 BHLHE41
Gene ID (NCBI) 79365
RRIDAB_2065361
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9C0J9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BHLHE41 (basic helix-loop-helix family member e41), also called SHARP1 and DEC2, is a member of the BHLH family proteins that are involved in differentiation, circadian rhythms and the response to hypoxia. In esophageal, osteosarcomas, prostate, breast, and endometrial cancer cells, BHLHE41 functions as an anti-apoptotic factor and promotes cell proliferation and invasiveness. It suppresses cell proliferation and metastasis, and also facilitates pancreatic as well as gastric cancer cell apoptosis. BHLHE41 has an important role in the proliferation of glioblastoma and couldserve as a novel candidate for targeted therapy of glioblastoma (PMID: 31616182).

实验方案

Product Specific Protocols
WB protocol for BHLHE41 antibody 12688-1-APDownload protocol
IHC protocol for BHLHE41 antibody 12688-1-APDownload protocol
IF protocol for BHLHE41 antibody 12688-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanchIP

Nat Commun

The basic helix-loop-helix transcription factor SHARP1 is an oncogenic driver in MLL-AF6 acute myelogenous leukemia.

Authors - Akihiko Numata
ratWB

FASEB J

Persistent myopathy despite release of partial obstruction: in vivo reversal of dysfunction and transcriptional responses using rapamycin.

Authors - Annette Schröder
humanWB

Biochem Biophys Res Commun

Loss of circadian protein TIMELESS accelerates the progression of cellular senescence.

Authors - Xiaomeng Shen
humanWB, IHC

J Exp Clin Cancer Res

DEC2 expression is positively correlated with HIF-1 activation and the invasiveness of human osteosarcomas.

Authors - Tu Hu
humanWB,IHC

J Exp Clin Cancer Res

Inhibition of DEC2 is necessary for exiting cell dormancy in salivary adenoid cystic carcinoma.

Authors - Xiao Yang
humanWB,IF

Cell Death Dis

Nanog mediated by FAO/ACLY signaling induces cellular dormancy in colorectal cancer cells.

Authors - Meng Zhang
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