验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, rat testis tissue |
| Positive IHC detected in | human ovary tumor tissue, mouse brain tissue, rat heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12688-1-AP targets BHLHE41 in WB, IHC, IF/ICC, IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, chIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | BHLHE41 fusion protein Ag3381 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | basic helix-loop-helix family, member e41 |
| 别名 | BHLHB3, Class B basic helix-loop-helix protein 3, Class E basic helix-loop-helix protein 41, DEC2, Enhancer-of-split and hairy-related protein 1 |
| 计算分子量 | 482 aa, 51 kDa |
| 观测分子量 | 50-52 kDa |
| GenBank蛋白编号 | BC025968 |
| 基因名称 | BHLHE41 |
| Gene ID (NCBI) | 79365 |
| RRID | AB_2065361 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9C0J9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BHLHE41 (basic helix-loop-helix family member e41), also called SHARP1 and DEC2, is a member of the BHLH family proteins that are involved in differentiation, circadian rhythms and the response to hypoxia. In esophageal, osteosarcomas, prostate, breast, and endometrial cancer cells, BHLHE41 functions as an anti-apoptotic factor and promotes cell proliferation and invasiveness. It suppresses cell proliferation and metastasis, and also facilitates pancreatic as well as gastric cancer cell apoptosis. BHLHE41 has an important role in the proliferation of glioblastoma and couldserve as a novel candidate for targeted therapy of glioblastoma (PMID: 31616182).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BHLHE41 antibody 12688-1-AP | Download protocol |
| IHC protocol for BHLHE41 antibody 12688-1-AP | Download protocol |
| IF protocol for BHLHE41 antibody 12688-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun The basic helix-loop-helix transcription factor SHARP1 is an oncogenic driver in MLL-AF6 acute myelogenous leukemia. | ||
FASEB J Persistent myopathy despite release of partial obstruction: in vivo reversal of dysfunction and transcriptional responses using rapamycin. | ||
Biochem Biophys Res Commun Loss of circadian protein TIMELESS accelerates the progression of cellular senescence. | ||
J Exp Clin Cancer Res DEC2 expression is positively correlated with HIF-1 activation and the invasiveness of human osteosarcomas. | ||
J Exp Clin Cancer Res Inhibition of DEC2 is necessary for exiting cell dormancy in salivary adenoid cystic carcinoma. | ||
Cell Death Dis Nanog mediated by FAO/ACLY signaling induces cellular dormancy in colorectal cancer cells. |
