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BHLHE22 Polyclonal antibody

BHLHE22 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 25622-1-AP

Print datasheet

Synonyms

Beta3, BHLHB5, BHLHE22, CAGL85, TNRC20

验证数据展示

  • WB analysis of SH-SY5Y using 25622-1-AP

    WB analysis of SH-SY5Y using 25622-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25622-1-AP (BHLHE22 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 25622-1-AP

    IHC staining of human brain using 25622-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25622-1-AP (BHLHE22 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of SH-SY5Y using 25622-1-AP

    IF Staining of SH-SY5Y using 25622-1-AP

    Immunofluorescent analysis of SH-SY5Y cells using 25622-1-AP (BHLHE22 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inSH-SY5Y cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

25622-1-AP targets BHLHE22 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitymouse, goat
Immunogen BHLHE22 fusion protein Ag20966 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name basic helix-loop-helix family, member e22
Synonyms Beta3, BHLHB5, BHLHE22, CAGL85, TNRC20
Calculated Molecular Weight 381 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC156671
Gene Symbol BHLHE22
Gene ID (NCBI) 27319
RRIDAB_2880165
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFJ8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Basic helix-loop-helix family member e22 (BHLHE22), a basic helix loop helix transcription factor family member, acts as a transcriptional repressor and has a role in cell differentiation in neuron development (PMID: 36941015). BHLHE22 expression serves as a potential biomarker for ICI treatment prediction and favorable prognosis, and that is associated with microenvironment immune status (PMID: 35806162).

实验方案

Product Specific Protocols
WB protocol for BHLHE22 antibody 25622-1-APDownload protocol
IHC protocol for BHLHE22 antibody 25622-1-APDownload protocol
IF protocol for BHLHE22 antibody 25622-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Metab

Deubiquitinase OTUD3 regulates metabolism homeostasis in response to nutritional stresses.

Authors - Na Zhou
View Article
goatWB

PLoS One

Chi-miR-4110 promotes granulosa cell apoptosis by targeting Sma- and Mad-related protein 2 (Smad2) in the caprine ovary.

Authors - Xiaopeng An
View Article