验证数据展示
经过测试的应用
Positive WB detected in | BxPC-3 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68279-1-Ig targets BDH2 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
经测试反应性 | Human |
免疫原 | BDH2 fusion protein Ag26108 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | 3-hydroxybutyrate dehydrogenase, type 2 |
别名 | BDH2, DHRS6, EFA6R, FLJ13261, Oxidoreductase UCPA, PRO20933, SDR15C1, UCPA OR, UNQ6308 |
观测分子量 | 27 kDa |
GenBank蛋白编号 | BC095414 |
基因名称 | BDH2 |
Gene ID (NCBI) | 56898 |
RRID | AB_2935360 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | Q9BUT1 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cytosolic type 2-hydroxybutyrate dehydrogenase, BDH2, originally named DHRS6, plays a physiological role in the cytosolic utilization of ketone bodies, which can subsequently enter mitochondria and the tricarboxylic acid cycle (PMID: 16380372). Moreover, BDH2 catalyzes the production of 2, 3-DHBA during enterobactin biosynthesis and participates in 24p3 (LCN2)-mediated iron transport and apoptosis (PMID: 23150981). It has 3 isoforms with molecular weights of 27, 19, and 16 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for BDH2 antibody 68279-1-Ig | Download protocol |
IF protocol for BDH2 antibody 68279-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |