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验证数据展示

  • WB analysis using 14324-1-AP (same clone as 14324-1-PBS)

    WB analysis using 14324-1-AP (same clone as 14324-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14324-1-AP (BCO2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14324-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate hyperplasia using 14324-1-AP (same clone as 14324-1-PBS)

    IHC staining of human prostate hyperplasia using 14324-1-AP (same clone as 14324-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 14324-1-AP (BCO2 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14324-1-PBS in a different storage buffer formulation.

BCO2 Polyclonal antibody, PBS Only

BCO2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14324-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

14324-1-PBS targets BCO2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5590

Product name: Recombinant human BCO2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 159-506 aa of BC041656

Sequence: ERFMSRFELPGKAAGFSYKVIRVPPEKVDLGETIHGVQVICSIASTEKGKPSYYHSFGMTRNYIIFIEQPLKMNLWKIATSKIRGKAFSDGISWEPQCNTRFHVVEKRTGQLLPGRYYSKPFVTFHQINAFEDQGCVIIDLCCQDNGRTLEVYQLQNLRKAGEGLDQVHNSAAKSFPRRFVLPLNVSLNAPEGDNLSPLSYTSASAVKQADGTIWCSHENLHQEDLEKEGGIEFPQIYYDRFSGKKYHFFYGCGFRHLVGDSLIKVDVVNKTLKVWREDGFYPSEPVFVPAPGTNEEDGGVILSVVITPNQNESNFLLVLDAKNFEELGRAEVPVQMPYGFHGTFIPI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 beta-carotene oxygenase 2
别名
计算分子量 66 kDa
观测分子量 60-65 kDa
GenBank蛋白编号BC041656
基因名称 BCO2
Gene ID (NCBI) 83875
RRIDAB_2227901
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BYV7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Beta,beta-carotene 9',10'-oxygenase (BCO2) catalyzes the asymmetric oxidative cleavage of beta-carotene in carotene metabolism.The apocarotenals formed by this enzyme may be the precursors for the biosynthesis of retinoic acid or exert unknown physiological effects. BCDO2 plays an important role in protecting against carotenoid-induced mitochondrial dysfunction and has a broader substrate specificity than previously recognized (PMID:22147584). This antibody detects the N-terminal of BCO2.

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