BCO2 Polyclonal antibody

BCO2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14324-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

B DIOX II, BCO2, Beta carotene dioxygenase 2, beta carotene oxygenase 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse liver tissue, L02 cells, rat liver tissue
Positive IHC detected inhuman prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14324-1-AP targets BCO2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 BCO2 fusion protein Ag5590 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 beta-carotene oxygenase 2
别名 B DIOX II, BCO2, Beta carotene dioxygenase 2, beta carotene oxygenase 2
计算分子量 66 kDa
观测分子量 60-65 kDa
GenBank蛋白编号BC041656
基因名称 BCO2
Gene ID (NCBI) 83875
RRIDAB_2227901
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BYV7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta,beta-carotene 9',10'-oxygenase (BCO2) catalyzes the asymmetric oxidative cleavage of beta-carotene in carotene metabolism.The apocarotenals formed by this enzyme may be the precursors for the biosynthesis of retinoic acid or exert unknown physiological effects. BCDO2 plays an important role in protecting against carotenoid-induced mitochondrial dysfunction and has a broader substrate specificity than previously recognized (PMID:22147584). This antibody detects the N-terminal of BCO2.

实验方案

Product Specific Protocols
WB protocol for BCO2 antibody 14324-1-APDownload protocol
IHC protocol for BCO2 antibody 14324-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human, mouseWB,IHC

Proc Natl Acad Sci U S A

Inactivity of human β,β-carotene-9',10'-dioxygenase (BCO2) underlies retinal accumulation of the human macular carotenoid pigment.

Authors - Binxing Li
mouseWB

J Nutr Biochem

Ablation of β,β-carotene-9',10'-oxygenase 2 remodels the hypothalamic metabolome leading to metabolic disorders in mice.

Authors - Xin Guo
mouseWB

Mol Nutr Food Res

Dietary wolfberry upregulates carotenoid metabolic genes and enhances mitochondrial biogenesis in the retina of db/db diabetic mice.

Authors - Yu Huifeng H
mouseWB

Mol Nutr Food Res

Wolfberries potentiate mitophagy and enhance mitochondrial biogenesis leading to prevention of hepatic steatosis in obese mice: The role of AMP-activated protein kinase α2 subunit.

Authors - Dingbo Lin
mouse

Molecules

Carotenoid Lutein Selectively Inhibits Breast Cancer Cell Growth and Potentiates the Effect of Chemotherapeutic Agents through ROS-Mediated Mechanisms.

Authors - Xiaoming Gong
humanWB,IF

Exp Eye Res

Imaging Macular Carotenoids and Their Related Proteins in the Human Retina with Confocal Resonance Raman and Fluorescence Microscopy

Authors - Binxing Li
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