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验证数据展示

  • WB analysis using 32103-1-AP

    WB analysis using 32103-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32103-1-AP (BCL9L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 32103-1-AP

    IF Staining of MCF-7 using 32103-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using BCL9L antibody (32103-1-AP) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

BCL9L Polyclonal antibody

BCL9L Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32103-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Nuclear Co-Factor Of Beta-Catenin Signalling, DLNB11, BCL9-like protein, B-cell lymphoma 9-like protein, B-cell CLL/lymphoma 9-like protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, MCF-7 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32103-1-AP targets BCL9L in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 BCL9L fusion protein Ag36794 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 B-cell CLL/lymphoma 9-like
别名 Nuclear Co-Factor Of Beta-Catenin Signalling, DLNB11, BCL9-like protein, B-cell lymphoma 9-like protein, B-cell CLL/lymphoma 9-like protein
计算分子量157 kDa
观测分子量220 kDa
GenBank蛋白编号NM_182557
基因名称 BCL9L
Gene ID (NCBI) 283149
RRIDAB_3670192
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ86UU0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

B-cell CLL/lymphoma 9-like protein (BCL9L, also known as B9L, BCL9-2, and DLNB11) is a transcriptional regulator that acts as an activator, which locates in nucleolus and nucleoplasm (PMID: 33767438). It is predicted to be implicated in the WNT-β-catenin signaling pathway to regulate the progress of hepatocellular carcinoma, breast cancer, and colon cancer (PMID: 31440992; 34545187; 30698996).

实验方案

Product Specific Protocols
WB protocol for BCL9L antibody 32103-1-APDownload protocol
IF protocol for BCL9L antibody 32103-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols