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验证数据展示

  • ChIP experiment of Raji using 81754-1-RR (same clone as 81754-1-PBS)

    ChIP experiment of Raji using 81754-1-RR (same clone as 81754-1-PBS)

    Chromatin was prepared from Raji cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of BCL6 (81754-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

  • WB analysis of Daudi using 81754-1-RR (same clone as 81754-1-PBS)

    WB analysis of Daudi using 81754-1-RR (same clone as 81754-1-PBS)

    WB result of BCL6 antibody (81754-1-RR; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCL6 transfected Daudi cells. This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

  • WB analysis using 81754-1-RR (same clone as 81754-1-PBS)

    WB analysis using 81754-1-RR (same clone as 81754-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81754-1-RR (BCL6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 81754-1-RR (same clone as 81754-1-PBS)

    IHC staining of human tonsillitis using 81754-1-RR (same clone as 81754-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 81754-1-RR (BCL6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 81754-1-RR (same clone as 81754-1-PBS)

    IHC staining of human tonsillitis using 81754-1-RR (same clone as 81754-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 81754-1-RR (BCL6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

  • IP experiment of Ramos using 81754-1-RR (same clone as 81754-1-PBS)

    IP experiment of Ramos using 81754-1-RR (same clone as 81754-1-PBS)

    IP result of anti-BCL6 (IP:81754-1-RR, 4ug; Detection:81754-1-RR 1:8000) with Ramos cells lysate 1680 ug. This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

  • FC experiment of Ramos using 81754-1-RR (same clone as 81754-1-PBS)

    FC experiment of Ramos using 81754-1-RR (same clone as 81754-1-PBS)

    1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human BCL6 (81754-1-RR, Clone:3K6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 81754-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81754-1-RR

    Affinity and Kinetic Characterization of 81754-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81754-1-RR against Human BCL6 were performed. The affinity constant is 0.157 nM.

  • Featured Product
  • KD/KO验证

BCL6 Recombinant monoclonal antibody, PBS Only

BCL6 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA, ChIP-qPCR
Cat No. 81754-1-PBS

产品说明书

CloneNo. 3K6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), IP, Indirect ELISA, ChIP-qPCR

别名

BCL-6, 3K6, B cell CLL/lymphoma 6, B cell lymphoma 6 protein, B-cell lymphoma 5 protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

81754-1-PBS targets BCL6 in WB, IHC, FC (Intra), IP, Indirect ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), IP, Indirect ELISA, ChIP-qPCR Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag15519

Product name: Recombinant human BCL6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 183-532 aa of BC150184

Sequence: SLYSGLSTPPASYSMYSHLPVSSLLFSDEEFRDVRMPVANPFPKERALPCDSARPVPGEYSRPTLEVSPNVCHSNIYSPKETIPEEARSDMHYSVAEGLKPAAPSARNAPYFPCDKASKEEERPSSEDEIALHFEPPNAPLNRKGLVSPQSPQKSDCQPNSPTESCSSKNACILQASGSPPAKSPTDPKACNWKKYKFIVLNSLNQNAKPEGPEQAELGRLSPRAYTAPPACQPPMEPENLDLQSPTKLSASGEDSTIPQASRLNNIVNRSMTGSPRSSSESHSPLYMHPPKCTSCGSQSPQHAEMCLHTAGPTFPEEMGETQSEYSDSSCENGAFFCNECDCRFSEEAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 B-cell CLL/lymphoma 6
别名 BCL-6, 3K6, B cell CLL/lymphoma 6, B cell lymphoma 6 protein, B-cell lymphoma 5 protein
计算分子量 706 aa, 79 kDa
观测分子量 79-95 kDa
GenBank蛋白编号BC150184
基因名称 BCL6
Gene ID (NCBI) 604
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP41182
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BCL6, a zinc finger transcription factor, contains an N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages.

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