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验证数据展示

  • WB analysis of HeLa using 23959-1-AP (same clone as 23959-1-PBS)

    WB analysis of HeLa using 23959-1-AP (same clone as 23959-1-PBS)

    WB result of BCL3 antibody (23959-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCL3 transfected HeLa cells. This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 23959-1-AP (same clone as 23959-1-PBS)

    IF Staining of HepG2 using 23959-1-AP (same clone as 23959-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BCL3 antibody (23959-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 23959-1-AP (same clone as 23959-1-PBS)

    WB analysis of HEK-293 using 23959-1-AP (same clone as 23959-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23959-1-AP (BCL3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 23959-1-AP (same clone as 23959-1-PBS)

    IP experiment of HEK-293 using 23959-1-AP (same clone as 23959-1-PBS)

    IP result of anti-BCL3 (IP:23959-1-AP, 4ug; Detection:23959-1-AP 1:600) with HEK-293 cells lysate 3000ug. This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 23959-1-AP (same clone as 23959-1-PBS)

    IHC staining of human lymphoma using 23959-1-AP (same clone as 23959-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

BCL3 Polyclonal antibody, PBS Only

BCL3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 23959-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

B-cell lymphoma 3 protein, BCL-3, BCL4, D19S37, Proto-oncogene BCL3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

23959-1-PBS targets BCL3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag21040

Product name: Recombinant human BCL3 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 97-446 aa of BC064993

Sequence: NLPTPLYPMMCPMEHPLSADIAMATRADEDGDTPLHIAVVQGNLPAVHRLVNLFQQGGRELDIYNNLRQTPLHLAVITTLPSVVRLLVTAGASPMALDRHGQTAAHLACEHRSPTCLRALLDSAAPGTLDLEARNYDGLTALHVAVNTECQETVQLLLERGADIDAVDIKSGRSPLIHAVENNSLSMVQLLLQHGANVNAQMYSGSSALHSASGRGLLPLVRTLVRSGADSSLKNCHNDTPLMVARSRRVIDILRGKATRPASTSQPDPSPDRSANTSPESSSRLSSNGLLSASPSSSPSQSPPRDPPGFPMAPPNFFLPSPSPPAFLPFAGVLRGPGRPVPPSPAPGGS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 B-cell CLL/lymphoma 3
别名 B-cell lymphoma 3 protein, BCL-3, BCL4, D19S37, Proto-oncogene BCL3
计算分子量 446 aa, 47 kDa
观测分子量 48-60 kDa
GenBank蛋白编号BC064993
基因名称 BCL3
Gene ID (NCBI) 602
RRIDAB_2879375
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20749
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BCL3 (B-cell lymphoma 3 protein) has seven ankyrin repeat domains, a proline-rich N terminal domain and a serine- and proline-rich C-terminal domain(PMID: 31930327). BCL3 is an important contributor to chronic inflammatory disease states, such as osteoarthritis and rheumatoid arthritis (PMID: 12483727). BCL3 is a predominantly nuclear protein containing a trans-activation domain, and it can be recruited to NF-κB-responsive promoters, resulting in transcriptional activation or repression depending on the subunit composition of NF-κB complexes (PMID: 25089799, PMID: 30135206). BCL3 is known to be overexpressed in human breast cancers and is associated with reduced metastasis-free survival (PMID: 38255248). The predicted molecular weight of the BCL3 protein is 48 kDa. However, due to post-translational modifications (e.g., phosphorylation), its apparent molecular weight often ranges between 55 and 65 kDa (PMID: 9407099, PMID: 12847243).

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