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验证数据展示

  • WB analysis using 55414-1-AP (same clone as 55414-1-PBS)

    WB analysis using 55414-1-AP (same clone as 55414-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55414-1-AP (BCL11B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55414-1-PBS in a different storage buffer formulation.

  • IP experiment of Jurkat using 55414-1-AP (same clone as 55414-1-PBS)

    IP experiment of Jurkat using 55414-1-AP (same clone as 55414-1-PBS)

    IP result of anti-BCL11B (IP:55414-1-AP, 4ug; Detection:55414-1-AP 1:5000) with Jurkat cells lysate 1320 ug. This data was developed using the same antibody clone with 55414-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 55414-1-AP (same clone as 55414-1-PBS)

    IHC staining of mouse brain using 55414-1-AP (same clone as 55414-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55414-1-AP (BCL11B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55414-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 55414-1-AP (same clone as 55414-1-PBS)

    IF Staining of HepG2 using 55414-1-AP (same clone as 55414-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 55414-1-AP (BCL11B antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 55414-1-PBS in a different storage buffer formulation.

BCL11B Polyclonal antibody, PBS Only

BCL11B Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 55414-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

COUP TF interacting protein 2, BCL-11B, B-cell lymphoma/leukemia 11B, B-cell CLL/lymphoma 11B, B cell lymphoma/leukemia 11B

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

55414-1-PBS targets BCL11B in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 B-cell CLL/lymphoma 11B (zinc finger protein)
别名 COUP TF interacting protein 2, BCL-11B, B-cell lymphoma/leukemia 11B, B-cell CLL/lymphoma 11B, B cell lymphoma/leukemia 11B
计算分子量 96 kDa
观测分子量 116 kDa
GenBank蛋白编号NM_022898
基因名称 BCL11B
Gene ID (NCBI) 64919
RRIDAB_11182609
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9C0K0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BCL11B, also named CTIP2, RIT1, CTIP-2, ZNF856B, and hRIT1-alpha, is a tumor-suppressor protein involved in T-cell lymphomas. It may function on the P53-signaling pathway. BCL11B is a key regulator of both differentiation and survival during thymocyte development. It depresses transcription through direct, TFCOUP2-independent binding to a GC-rich response element. This antibody is specific to BCL11B. The calculated molecular weight of BCL11B is 95 kDa but the modified BCL11B protein is about 116 kDa.

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