Various lysates were subjected to SDS PAGE followed by western blot with 85858-2-RR (BCL10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85858-2-PBS in a different storage buffer formulation.

The mean BCL10 concentration was determined to be 105.67 ng/mL in Jurkat cell extract based on a 1.20 mg/mL extract load, 327.67 ng/mL in K-562 cell extract based on a 3.00 mg/mL extract load and 82.11 ng/mL in A549 cell extract based on a 1.10 mg/mL extract load.

Sandwich ELISA standard curve of MP02160-2, Human BCL10 Recombinant Matched Antibody Pair - PBS only. 85858-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag12170. 85858-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

Cytometric bead array standard curve of MP02160-2, BCL10 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85858-3-PBS. Detection antibody: 85858-2-PBS. Standard: Ag12170. Range: 0.781-100 ng/mL.

Biolayer interferometry (BLl) kinetic assays of 85858-2-RR against Human BCL10 were performed. The affinity constant is below 1 pM.