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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 85799-3-RR (same clone as 85799-3-PBS)

    WB analysis using 85799-3-RR (same clone as 85799-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85799-3-RR (BCKDHA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85799-3-PBS in a different storage buffer formulation.

  • WB analysis using 85799-3-RR (same clone as 85799-3-PBS)

    WB analysis using 85799-3-RR (same clone as 85799-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85799-3-RR (BCKDHA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85799-3-PBS in a different storage buffer formulation.

BCKDHA Recombinant antibody, PBS Only

BCKDHA Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85799-3-PBS

产品说明书

CloneNo. 250051G11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial, BCKDH E1-alpha, Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain, EC:1.2.4.4, MSU

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy BCKDHA Ready-To-Use IHC Kit. BCKDHA primary antibody included.

产品信息

85799-3-PBS targets BCKDHA in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 BCKDHA fusion protein Ag32613 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 branched chain keto acid dehydrogenase E1, alpha polypeptide
别名 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial, BCKDH E1-alpha, Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain, EC:1.2.4.4, MSU
计算分子量 50 kDa
观测分子量42-50 kDa
GenBank蛋白编号BC008933
基因名称 BCKDHA
Gene ID (NCBI) 593
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP12694
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), the gene encoding the regulated subunit of BCKDC was only one of two primary susceptibility genes identified that affected the risk of both type 2 diabetes mellitus (T2DM) and obesity (PMID: 25287287). BIX01294 transcriptionally downregulated the transcription of BCKDHA, which is essential for fueling the tricarboxylic acid (TCA) cycle. Studies have shown that KDM3A, a Jumonji histone demethylase, epigenetically regulates BCKDHA expression by binding to the BCKDHA gene promoter (PMID: 34876693). Moreover, at least four genes including BCKDHA, branched chain keto acid dehydrogenase E1, beta polypeptide (BCKDHB), dihydrolipoamide dehydrogenase (DLD), and dihydrolipoamide branched chain transacylase E2 (DBT) have been reported to be the causative gene for Maple syrup urine disease (MSUD) (PMID: 34187135).