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验证数据展示

  • WB analysis using 16815-1-AP

    WB analysis using 16815-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16815-1-AP (p130Cas / BCAR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 16815-1-AP

    IHC staining of human colon cancer using 16815-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16815-1-AP (p130Cas / BCAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 16815-1-AP

    IHC staining of human colon cancer using 16815-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16815-1-AP (p130Cas / BCAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of RAW 264.7 using 16815-1-AP

    IP experiment of RAW 264.7 using 16815-1-AP

    IP result of anti-p130Cas / BCAR1 (IP:16815-1-AP, 4ug; Detection:16815-1-AP 1:600) with RAW 264.7 cells lysate 4000ug.

  • FC experiment of A431 using 16815-1-AP

    FC experiment of A431 using 16815-1-AP

    1x10^6 A431 cells were intracellularly stained with 0.25 ug p130Cas / BCAR1 Polyclonal antibody (16815-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IF Staining of HeLa using 16815-1-AP

    IF Staining of HeLa using 16815-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using p130Cas / BCAR1 antibody (16815-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

p130Cas / BCAR1 Polyclonal antibody

p130Cas / BCAR1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 16815-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

别名

BCAR1, Breast cancer anti-estrogen resistance protein 1, CAS, CAS1, CASS1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, A431 cells, HepG2 cells, T-47D cells
Positive IP detected inRAW 264.7 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16815-1-AP targets p130Cas / BCAR1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10354

Product name: Recombinant human BCAR1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-420 aa of BC062556

Sequence: MNHLNVLAKALYDNVAESPDELSFRKGDIMTVLEQDTQGLDGWWLCSLHGRQGIVPGNRLKILVGMYDKKPAGPGSGPPATPAQPQPGLHAPAPPASQYTPMLPNTYQPQPDSVYLVPTPSKAQQGLYQVPGPSPQFQSPPAKQTSTFSKQTPHHPFPNPATDLYQVPPGPGGPAQDIYQVPPSAGMGHDIYQVPPSMDTRSWEGTKPPAKVVVPTRVGQGYVYEAAQPEQDEYDIPRHLLAPGPQDIYDVPPVRGLLPSQYGQEVYDTPPMAVKGPNGRDPLLEVYDVPPSVEKGLPPSNHHAVYDVPPSVSKDVPDGPLLREETYDVPPAFAKAKPFDPARTPLVLAAPPPDSPPAEDVYDVPPPAPDLYDVPPGLRRPGPGTLYDVPRERVLPPEVADGGVVDSGVYAVPPPAEREA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 breast cancer anti-estrogen resistance 1
别名 BCAR1, Breast cancer anti-estrogen resistance protein 1, CAS, CAS1, CASS1
计算分子量 870 aa, 93 kDa
观测分子量 116 kDa
GenBank蛋白编号BC062556
基因名称 BCAR1
Gene ID (NCBI) 9564
RRIDAB_2065460
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP56945
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for p130Cas / BCAR1 antibody 16815-1-APDownload protocol
IF protocol for p130Cas / BCAR1 antibody 16815-1-APDownload protocol
IHC protocol for p130Cas / BCAR1 antibody 16815-1-APDownload protocol
IP protocol for p130Cas / BCAR1 antibody 16815-1-APDownload protocol
WB protocol for p130Cas / BCAR1 antibody 16815-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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