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验证数据展示

  • WB analysis using 84584-5-RR (same clone as 84584-5-PBS)

    WB analysis using 84584-5-RR (same clone as 84584-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84584-5-RR (BAP31 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84584-5-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 84584-5-RR (same clone as 84584-5-PBS)

    IHC staining of human colon cancer using 84584-5-RR (same clone as 84584-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84584-5-RR (BAP31 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84584-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BCAP31 antibody included
  • IHC staining of human colon cancer using 84584-5-RR (same clone as 84584-5-PBS)

    IHC staining of human colon cancer using 84584-5-RR (same clone as 84584-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84584-5-RR (BAP31 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84584-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BCAP31 antibody included
  • IF Staining of HeLa using 84584-5-RR (same clone as 84584-5-PBS)

    IF Staining of HeLa using 84584-5-RR (same clone as 84584-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BAP31 antibody (84584-5-RR, Clone: 241978D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84584-5-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 84584-5-RR (same clone as 84584-5-PBS)

    FC experiment of HepG2 using 84584-5-RR (same clone as 84584-5-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug BAP31 Recombinant antibody (84584-5-RR, Clone:241978D10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84584-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84584-5-RR

    Affinity and Kinetic Characterization of 84584-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84584-5-RR against Human BAP31 were performed. The affinity constant is below 1 pM.

BAP31 Recombinant monoclonal antibody, PBS Only

BAP31 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84584-5-PBS

产品说明书

CloneNo. 241978D10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

BCAP31, 241978D10, 6C6 AG, 6C6-AG tumor-associated antigen, B-cell receptor-associated protein 31

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy BCAP31 Ready-To-Use IHC Kit. BCAP31 primary antibody included.

产品信息

84584-5-PBS targets BAP31 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1668

Product name: Recombinant human BCAP31 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-246 aa of BC014323

Sequence: MSLQWTAVATFLYAEVFVVLLLCIPFISPKRWQKIFKSRLVELLVSYGNTFFVVLIVILVLLVIDAVREIREYDDVTEKVNLQNNPGAMEHFHMKLFRAQRNLYIAGFSLLLSFLLRRLVTLISQQATLLASNEAFKKQAESASEAAKKYMEENDQLKKGAAVDGGKLDVGNAEVKLEEENRSLKADLQKLKDELASTKQKLEKAENQVLAMRKQSEGLTKEYDRLLEEHAKLQAAVDGPMDKKEE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 B-cell receptor-associated protein 31
别名 BCAP31, 241978D10, 6C6 AG, 6C6-AG tumor-associated antigen, B-cell receptor-associated protein 31
计算分子量 28 kDa
观测分子量 28 kDa
GenBank蛋白编号BC014323
基因名称 BAP31
Gene ID (NCBI) 10134
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP51572
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BAP31, also known as BCAP31, is a chaperone protein abundant in the endoplasmic reticulum (ER). BAP31 plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded proteins, and their targeting to the ER-associated-degradation (ERAD). CDIP1 and BAP31 interact upon ER stress to regulate the mitochondrial apoptosis pathway(PMID: 24139803). It also serves as a cargo receptor for the export of transmembrane proteins. BAP31 may be involved in apoptosis.

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