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验证数据展示

  • WB analysis using 12766-1-AP (same clone as 12766-1-PBS)

    WB analysis using 12766-1-AP (same clone as 12766-1-PBS)

    HEK-293 cell lysates were subjected to SDS PAGE followed by western blot with 12766-1-AP (BBS4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12766-1-PBS in a different storage buffer formulation.

  • IF Staining of hTERT-RPE1 cells using 12766-1-AP (same clone as 12766-1-PBS)

    IF Staining of hTERT-RPE1 cells using 12766-1-AP (same clone as 12766-1-PBS)

    IF result (cytoplasm and the length of cilia cilia stain) of anti-BBS4 (12766-1-AP; 1:50) with serum-starved hTERT-RPE1 cell (MeOH fixed) by Dr. Moshe Kim. This data was developed using the same antibody clone with 12766-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse stomach using 12766-1-AP (same clone as 12766-1-PBS)

    IHC staining of mouse stomach using 12766-1-AP (same clone as 12766-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12766-1-AP (BBS4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12766-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BBS4 antibody included
  • IHC staining of rat kidney using 12766-1-AP (same clone as 12766-1-PBS)

    IHC staining of rat kidney using 12766-1-AP (same clone as 12766-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 12766-1-AP (BBS4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12766-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BBS4 antibody included
  • IF Staining of hTERT-RPE1 using 12766-1-AP (same clone as 12766-1-PBS)

    IF Staining of hTERT-RPE1 using 12766-1-AP (same clone as 12766-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using BBS4 antibody (12766-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 12766-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

BBS4 Polyclonal antibody, PBS Only

BBS4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12766-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Bardet Biedl syndrome 4, Bardet-Biedl syndrome 4 protein

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy BBS4 Ready-To-Use IHC Kit. BBS4 primary antibody included.

产品信息

12766-1-PBS targets BBS4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3430

Product name: Recombinant human BBS4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC027624

Sequence: MAEERVATRTQFPVSTESQKPRQKKAPEFPILEKQNWLIHLHYIRKDYEACKAVIKEQLQETQGLCEYAIYVQALIFRLEGNIQESLELFQTCAVLSPQSADNLKQVARSLFLLGKHKAAIEVYNEAAKLNQKDWEISHNLGVCYIYLKQFNKAQDQLHNALNLNRHDLTYIMLGKIHLLEGDLDKAIEVYKKAVEFSPENTELLTTLGLLYLQLGIYQKAFEHLGNALTYDPTNYKAILAAGSMMQTHGDFDVALTKYRVVACAVPESPPLWNNIGMCFFGKKKYVAAISCLKRANYLAPFDWKILYNLGLVHLTMQQYASAFHFLSAAINFQPKMGELYMLLAVALTNL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Bardet-Biedl syndrome 4
别名 Bardet Biedl syndrome 4, Bardet-Biedl syndrome 4 protein
计算分子量 519 aa, 58 kDa
观测分子量 50-60 kDa
GenBank蛋白编号BC027624
基因名称 BBS4
Gene ID (NCBI) 585
RRIDAB_10596774
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96RK4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BBS4 (Bardet-Biedl syndrome protein 4) is one of the early BBS proteins discovered, with 35 mutations reported to cause the BBS phenotype (PMID: 26762677). BBS4 undergoes localization to the centriolar satellites of centrosomes and basal body of primary cilia, where it takes part in recruiting cargo to centriolar satellites and allowing the formation of a functional centrosomal microtubule organizing center (MTOC) (PMID: 25860617). Some research has found that BBS4 expression in humans during the development of CNS and sensory organs, concerning the cardinal clinical signs of BBS (PMID: 33860840).

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