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验证数据展示

  • WB analysis of HepG2 using 23976-1-AP

    WB analysis of HepG2 using 23976-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23976-1-AP (BARHL2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 23976-1-AP

    IHC staining of human testis using 23976-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23976-1-AP (BARHL2 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 23976-1-AP

    IF Staining of HepG2 using 23976-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BARHL2 antibody (23976-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

BARHL2 Polyclonal antibody

BARHL2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 23976-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

BarH like 2 homeobox protein, BarH like homeobox 2, BARHL2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, NIH/3T3 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23976-1-AP targets BARHL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 BARHL2 fusion protein Ag21156 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 BarH-like homeobox 2
别名 BarH like 2 homeobox protein, BarH like homeobox 2, BARHL2
计算分子量 387 aa, 42 kDa
观测分子量 42-45 kDa
GenBank蛋白编号BC126439
基因名称 BARHL2
Gene ID (NCBI) 343472
RRIDAB_2879386
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ9NY43
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BARHL2 also named as BarH like homeobox 2 is 279 amino acid protein, which contains 1 homeobox DNA-binding domain. BARHL2 localizes in nucleus and belongs to BAR homeobox family. BARHL2 as a transcription factor binds optimally to the DNA consensus sequence. Barx2 may differentially control the expression of L1 and other target genes during embryonic development. Barx2 is expressed in several smooth muscle-containing tissues, as well as skeletal muscle, brain, tongue and esophagus.

实验方案

Product Specific Protocols
WB protocol for BARHL2 antibody 23976-1-APDownload protocol
IHC protocol for BARHL2 antibody 23976-1-APDownload protocol
IF protocol for BARHL2 antibody 23976-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Nat Aging

Transcriptional and epigenetic dysregulation impairs generation of proliferative neural stem and progenitor cells during brain aging

Authors - Meiyang Li
View Article