验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, rat testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31236-1-AP targets BANF2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 |
CatNo: Ag35307 Product name: Recombinant human BANF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-90 aa of PL00285355 Sequence: MDNMSPRLRAFLSEPIGEKDVCWVDGISHELAINLVTKGINKAYILLGQFLLMHKNEAEFQRWLICCFGATECEAQQTSHCLKEWCACFL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | barrier to autointegration factor 2 |
| 别名 | BAF L, BAF Like, BAF2, BAFL, BANF2, C20orf179 |
| 计算分子量 | 10 kDa |
| 观测分子量 | 11 kDa |
| GenBank蛋白编号 | PL00285355 |
| 基因名称 | BANF2 |
| Gene ID (NCBI) | 140836 |
| RRID | AB_3669913 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9H503 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BANF2 (Barrier-to-autointegration factor 2), also called BAF-L (Barrier-to-autointegration factor-like protein), is a homologous gene termed BAF, which shows a 53% similarity in the protein sequence with BANF2 in humans (PMID: 35216101; 16337940). Unlike BAF, BANF2 plays an important role in spermiogenesis and spermatozoal function, and it is a male germ cell marker, whose expression is highly associated with the maturation of male germ cells (PMID: 33951625; 35216101).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BANF2 antibody 31236-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
