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验证数据展示

  • WB analysis using 83779-4-RR (same clone as 83779-4-PBS)

    WB analysis using 83779-4-RR (same clone as 83779-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83779-4-RR (BAG3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83779-4-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 83779-4-RR (same clone as 83779-4-PBS)

    IHC staining of human stomach cancer using 83779-4-RR (same clone as 83779-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83779-4-RR (BAG3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83779-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BAG3 antibody included
  • IF Staining of HepG2 using 83779-4-RR (same clone as 83779-4-PBS)

    IF Staining of HepG2 using 83779-4-RR (same clone as 83779-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BAG3 antibody (83779-4-RR, Clone: 240860F12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83779-4-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83779-4-RR (same clone as 83779-4-PBS)

    FC experiment of HeLa using 83779-4-RR (same clone as 83779-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug BAG3 Recombinant antibody (83779-4-RR, Clone:240860F12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83779-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83779-4-RR

    Affinity and Kinetic Characterization of 83779-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83779-4-RR against Human BAG3 were performed. The affinity constant is below 1 pM.

BAG3 Recombinant antibody, PBS Only

BAG3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83779-4-PBS

产品说明书

CloneNo. 240860F12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

Docking protein CAIR-1, BIS, Bcl-2-binding protein Bis, Bcl-2-associated athanogene 3, BAG-3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy BAG3 Ready-To-Use IHC Kit. BAG3 primary antibody included.

产品信息

83779-4-PBS targets BAG3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 BAG3 fusion protein Ag0956 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 BCL2-associated athanogene 3
别名 Docking protein CAIR-1, BIS, Bcl-2-binding protein Bis, Bcl-2-associated athanogene 3, BAG-3
计算分子量 61 kDa
观测分子量 74-80 kDa
GenBank蛋白编号BC006418
基因名称 BAG3
Gene ID (NCBI) 9531
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO95817
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BAG3 (Bcl2-associated athanogene 3) belongs to the BAG protein family, the co-chaperone that binds to Hsc70/Hsp70 through the BAG domain and modulates their activity in polypeptide folding. BAG3 contains also a WW domain and a proline-rich (PXXP) repeat, that mediate binding to partners different from Hsp70. Through interacting with different molecular partner, BAG3 influences several cell processes, such as apoptosis, autophagy and cell motility. BAG3 protein has been reported to sustain cell survival, resistance to therapy, and/or motility and metastatization in several tumor types, thus being identified as a potential target for anticancer therapies. In addition, defects in BAG3 are the cause of some myopathy. BAG3 normally migrates around 74-80 kDa; a slightly different molecular weight or a doublet form can be observed in some cell types and/or following cell exposure to stressors. A synaptosome associated form of 40 kDa has recently been described.