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验证数据展示

  • WB analysis using 83779-4-RR

    WB analysis using 83779-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83779-4-RR (BAG3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 83779-4-RR

    IHC staining of human stomach cancer using 83779-4-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83779-4-RR (BAG3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

    Ready-to-use IHC kits available with BAG3 antibody included
  • IF Staining of HepG2 using 83779-4-RR

    IF Staining of HepG2 using 83779-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BAG3 antibody (83779-4-RR, Clone: 240860F12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HeLa using 83779-4-RR

    FC experiment of HeLa using 83779-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug BAG3 Recombinant antibody (83779-4-RR, Clone:240860F12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 83779-4-RR

    Affinity and Kinetic Characterization of 83779-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83779-4-RR against Human BAG3 were performed. The affinity constant is below 1 pM.

BAG3 Recombinant monoclonal antibody

BAG3 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83779-4-RR

产品说明书

CloneNo. 240860F12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

240860F12, BAG-3, Bcl-2-associated athanogene 3, Bcl-2-binding protein Bis, BIS

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, K-562 cells, DU 145 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy BAG3 Ready-To-Use IHC Kit. BAG3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83779-4-RR targets BAG3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag0956

Product name: Recombinant human BAG3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 275-575 aa of BC006418

Sequence: SREGSPARSSTPLHSPSPIRVHTVVDRPQQPMTHRETAPVSQPENKPESKPGPVGPELPPGHIPIQVIRKEVDSKPVSQKPPPPSEKVEVKVPPAPVPCPPPSPGPSAVPSSPKSVATEERAAPSTAPAEATPPKPGEAEAPPKHPGVLKVEAILEKVQGLEQAVDNFEGKKTDKKYLMIEEYLTKELLALDSVDPEGRADVRQARRDGVRKVQTILEKLEQKAIDVPGQVQVYELQPSNLEADQPLQAIMEMGAVAADKGKKNAGNAEDPHTETQQPEATAAATSNPSSMTDTPGNPAAP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 BCL2-associated athanogene 3
别名 240860F12, BAG-3, Bcl-2-associated athanogene 3, Bcl-2-binding protein Bis, BIS
计算分子量 61 kDa
观测分子量 74-80 kDa
GenBank蛋白编号BC006418
基因名称 BAG3
Gene ID (NCBI) 9531
RRIDAB_3671372
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO95817
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BAG3 (Bcl2-associated athanogene 3) belongs to the BAG protein family, the co-chaperone that binds to Hsc70/Hsp70 through the BAG domain and modulates their activity in polypeptide folding. BAG3 contains also a WW domain and a proline-rich (PXXP) repeat, that mediate binding to partners different from Hsp70. Through interacting with different molecular partner, BAG3 influences several cell processes, such as apoptosis, autophagy and cell motility. BAG3 protein has been reported to sustain cell survival, resistance to therapy, and/or motility and metastatization in several tumor types, thus being identified as a potential target for anticancer therapies. In addition, defects in BAG3 are the cause of some myopathy. BAG3 normally migrates around 74-80 kDa; a slightly different molecular weight or a doublet form can be observed in some cell types and/or following cell exposure to stressors. A synaptosome associated form of 40 kDa has recently been described.

实验方案

Product Specific Protocols
FC protocol for BAG3 antibody 83779-4-RRDownload protocol
IF protocol for BAG3 antibody 83779-4-RRDownload protocol
IHC protocol for BAG3 antibody 83779-4-RRDownload protocol
WB protocol for BAG3 antibody 83779-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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