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验证数据展示

  • IF Staining of HeLa using CL488-19064

    IF Staining of HeLa using CL488-19064

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 BAG1 antibody (CL488-19064) at dilution of 1:200, CL594-Phalloidin (red).

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  • KD/KO验证

CoraLite® Plus 488-conjugated BAG1 Polyclonal antibody

BAG1 Polyclonal Antibody for IF/ICC
Cat No. CL488-19064

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC

别名

BAG 1, BAG1, BAG1L speicfic, BAG1M, BAG1S, HAP, RAP46

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 19064-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-19064 targets BAG1 in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 BAG1 fusion protein Ag13555 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 BCL2-associated athanogene
别名 BAG 1, BAG1, BAG1L speicfic, BAG1M, BAG1S, HAP, RAP46
计算分子量 39 kDa
观测分子量 50-55 kDa, 30-33 kDa
GenBank蛋白编号BC014774
基因名称 BAG1
Gene ID (NCBI) 573
RRIDAB_3672704
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99933
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BAG1 have been identified that modulate gene transcription through poorly defined mechanisms. Four isoforms of the BAG1 protein (BAG1S, BAG1, BAG1M and BAG1L) can be produced from a common mRNA by use of alternative translation initiation sites, including a non-canonical CTG codon in one instance. The longest, BAG1L (Mr ~50K), contains a nuclear localization signal (NLS) and resides in the nucleus, whereas BAG1M (Mr ~46K) has an incomplete NLS and distributes mainly in cytosol, unless dragged into the nucleus through interactions with other. Distribution of BAG1S(p33) is not clear yet. This antibody can recognize all the isoforms of BAG1.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 BAG1 antibody CL488-19064Download protocol
Standard Protocols
Click here to view our Standard Protocols