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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated BACH1 Monoclonal antibody

BACH1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, mouse, pig, rabbit

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D12A4

Cat No : CL594-66762

Print datasheet

Synonyms

BACH 1, BACH1, BTB and CNC homolog 1, HA2303

验证数据展示

  • FC experiment of K-562 using CL594-66762

    FC experiment of K-562 using CL594-66762

    1x10^6 K-562 cells were intracellularly stained with 0.8 ug CoraLite®594-conjugated BACH1 Monoclonal antibody (CL594-66762, Clone:2D12A4)(red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC detected inK-562 cells
For other applications, we recommend the unconjugated version of this antibody, 66762-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66762 targets BACH1 in FC (Intra) applications and shows reactivity with Human, Rat, mouse, pig, rabbit samples.

Tested Applications FC (Intra)
Tested Reactivity Human, Rat, mouse, pig, rabbit
Immunogen BACH1 fusion protein Ag6011 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name BTB and CNC homology 1, basic leucine zipper transcription factor 1
Synonyms BACH 1, BACH1, BTB and CNC homolog 1, HA2303
Calculated Molecular Weight 82 kDa
Observed Molecular Weight 100-110 kDa, 82 kDa
GenBank Accession NumberBC063307
Gene Symbol BACH1
Gene ID (NCBI) 571
ENSEMBL Gene IDENSG00000156273
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO14867
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BTB and CNC homology 1, basic leucine zipper transcription factor 1(BACH1) is a heme-regulated transcriptional regulator that acts as repressor or activator and a member of the AP-a superfamily. MafK belongs to the small Maf family that can funciton as transcriptional activators or repressors depending on the proteins with which they form heterdimers. BACH1 has crucial roles in both stress responses and transformation by H-RasV12. The molecular weight of expected mobility Bach1 is 100-110 kDa.

实验方案

Product Specific Protocols
FC protocol for CL594 BACH1 antibody CL594-66762Download protocol
Standard Protocols
Click here to view our Standard Protocols