验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, Hela cells, SH-SY5Y cells |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
16321-1-AP targets BACE2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag9404 Product name: Recombinant human BACE2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 39-398 aa of BC014453 Sequence: TNRVVAPTPGPGTPAERHADGLALALEPALASPAGAANFLAMVDNLQGDSGRGYYLEMLIGTPPQKLQILVDTGSSNFAVAGTPHSYIDTYFDTERSSTYRSKGFDVTVKYTQGSWTGFVGEDLVTIPKGFNTSFLVNIATIFESENFFLPGIKWNGILGLAYATLAKPSSSLETFFDSLVTQANIPNVFSMQMCGAGLPVAGSGTNGGSLVLGGIEPSLYKGDIWYTPIKEEWYYQIEILKLEIGGQSLNLDCREYNADKAIVDSGTTLLRLPQKVFDAVVEAVARASLIPEFSDGFWTGSQLACWTNSETPWSYFPKISIYLRDENSSRSFRITILPQLYIQPMMGAGLNYECYRFGI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | beta-site APP-cleaving enzyme 2 |
| 别名 | AEPLC, ALP56, Asp 1, ASP21, Aspartic-like protease 56 kDa |
| 计算分子量 | 518 aa, 56 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC014453 |
| 基因名称 | BACE2 |
| Gene ID (NCBI) | 25825 |
| RRID | AB_2061667 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y5Z0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BACE2, also known as ASP-1 or memapsin 1, is the closest homologue of memapsin 2, with 50% residue identity. BACE2 is located on chromosome 21 in the Down's syndrome (DS) obligate region and may contribute to amyloid abnormalities in these individuals. BACE2 transcripts are expressed in the central nervous system and many peripheral tissues; however, its expression level in neurons is substantially lower than BACE1.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for BACE2 antibody 16321-1-AP | Download protocol |
| WB protocol for BACE2 antibody 16321-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Brain Behav Immun Elevated β-secretase 1 expression mediates CD4+ T cell dysfunction via PGE2 signalling in Alzheimer's disease. | ||
Int J Neurosci Activating transcription factor 6 reduces Aβ1-42 and restores memory in Alzheimer's disease model mice. |
