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验证数据展示

  • WB analysis of HEK-293 using 27428-1-AP

    WB analysis of HEK-293 using 27428-1-AP

    WB result of B7-H6 antibody (27428-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-B7-H6 transfected HEK-293 cells.

  • WB analysis using 27428-1-AP

    WB analysis using 27428-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27428-1-AP (B7H6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

B7-H6 Polyclonal antibody

B7-H6 Polyclonal Antibody for WB, ELISA
Cat No. 27428-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

B7H6, NCR3LG1, B7 H6, B7 homolog 6, Natural cytotoxicity triggering receptor 3 ligand 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27428-1-AP targets B7-H6 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag26532

Product name: Recombinant human DKFZp686O24166 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 25-259 aa of BC136800

Sequence: DLKVEMMAGGTQITPLNDNVTIFCNIFYSQPLNITSMGITWFWKSLTFDKEVKVFEFFGDHQEAFRPGAIVSPWRLKSGDASLRLPGIQLEEAGEYRCEVVVTPLKAQGTVQLEVVASPASRLLLDQVGMKENEDKYMCESSGFYPEAINITWEKQTQKFPHPIEISEDVITGPTIKNMDGTFNVTSCLKLNSSQEDPGTVYQCVVRHASLHTPLRSNFTLTAARHSLSETEKTD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hypothetical protein DKFZp686O24166
别名 B7H6, NCR3LG1, B7 H6, B7 homolog 6, Natural cytotoxicity triggering receptor 3 ligand 1
计算分子量51 kDa
观测分子量75-80 kDa
GenBank蛋白编号BC136800
基因名称 B7H6
Gene ID (NCBI) 374383
RRIDAB_3085956
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ68D85
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

B7-H6, also known as NCR3LG1, is a member of the B7 family of immunoreceptors. It is a 454-aa-long type I transmembrane protein consisting of an extracellular region, a transmembrane segment, and a cytoplasmic domain which contains an immunoreceptor tyrosine-based inhibitory motif (ITIM), an SH2-binding domain, and an SH3-binding motif. B7-H6 triggers NKp30-mediated activation of human NK cells (PMID: 19528259). It has been reported that B7H6 is expressed in various types of tumor cells and plays a prominent role in natural killer cell immunosurveillance (PMID: 35697295). The apparent molecular weight of B7-H6 is larger than the calculated molecular weight of 51 kDa due to glycosylation (PMID: 19528259).

实验方案

Product Specific Protocols
WB protocol for B7-H6 antibody 27428-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols