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验证数据展示

  • WB analysis of mouse liver using 12080-1-AP (same clone as 12080-1-PBS)

    WB analysis of mouse liver using 12080-1-AP (same clone as 12080-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12080-1-AP (B7‑H4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 12080-1-AP (same clone as 12080-1-PBS)

    IHC staining of human ovary tumor using 12080-1-AP (same clone as 12080-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12080-1-AP (B7‑H4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VTCN1 antibody included

B7‑H4 Polyclonal antibody, PBS Only

B7‑H4 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12080-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

VTCN1, B7S1, B7-H4, B7H4, B7h.5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy VTCN1 Ready-To-Use IHC Kit. VTCN1 primary antibody included.

产品信息

12080-1-PBS targets B7‑H4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2712

Product name: Recombinant human VTCN1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-187 aa of BC065717

Sequence: MFRGRTAVFADQVIVGNASLRLKNVQLTDAGTYKCYIITSKGKGNANLEYKTGAFSMPEVNVDYNASSETLRCEAPRWFPQPTVVWASQVDQGANFSEVSNTSFELNSENVTMKVVSVLYNVTINNTYSCMIENDIAKATGDIKVTESEIKRRSHLQLLNSKASLCVSSFFAISWALLPLSPYLMLK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 V-set domain containing T cell activation inhibitor 1
别名 VTCN1, B7S1, B7-H4, B7H4, B7h.5
计算分子量 282 aa, 31 kDa
观测分子量 55-65 kDa
GenBank蛋白编号BC065717
基因名称 B7-H4
Gene ID (NCBI) 79679
RRIDAB_2877822
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z7D3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

B7‑H4 also named VTCN1, B7X, or B7S1 is a 282 amino acid protein, which contains 2 immunoglobulin-like domains and belongs to the immunoglobulin superfamily. B7‑H4 negatively regulates T-cell mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. B7‑H4 is a single-pass type I membrane protein, which is over-expressed in breast, ovarian, endometrial, renal cell and non-small-cell lung cancers. The predicted molecular weight of B7‑H4 is 31 kDa. The glycosylated B7‑H4 is 50 to 80 kDa, and the non-glycosylated form is 28 kDa.