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验证数据展示

  • WB analysis using 85916-1-RR (same clone as 85916-1-PBS)

    WB analysis using 85916-1-RR (same clone as 85916-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85916-1-RR (Atp1b1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85916-1-PBS in a different storage buffer formulation.

  • WB analysis using 85916-1-RR (same clone as 85916-1-PBS)

    WB analysis using 85916-1-RR (same clone as 85916-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85916-1-RR (Atp1b1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85916-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP02193-3

    Sandwich ELISA standard curve of MP02193-3

    Sandwich ELISA standard curve of MP02193-3, Mouse ATP1B1 Recombinant Matched Antibody Pair - PBS only. 85916-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3007. 85916-1-PBS was HRP conjugated as the detection antibody. Range: 46.9-3000 pg/mL.

  • Sample test of MP02193-3

    Sample test of MP02193-3

    The mean ATP1B1 concentration was determined to be 58,169.6 pg/mL in mouse heart extract based on a 6.9 mg/mL extract load.

  • IHC staining of mouse liver using 85916-1-RR (same clone as 85916-1-PBS)

    IHC staining of mouse liver using 85916-1-RR (same clone as 85916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85916-1-RR (ATP1B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85916-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 85916-1-RR (same clone as 85916-1-PBS)

    IHC staining of mouse brain using 85916-1-RR (same clone as 85916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85916-1-RR (ATP1B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85916-1-PBS in a different storage buffer formulation.

    {{ptg:ProductFeaturesInfo}}

Mouse ATP1B1 Recombinant monoclonal antibody, PBS Only (Detector)

ATP1B1 Uni-rAb® Recombinant Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85916-1-PBS

产品说明书

CloneNo. 243093E3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

别名

ATP1B 1, Atp4b, Beta 1 subunit of Na(+) K(+) ATPase, Na, K-ATPase β1, Na+/K+ ATPase beta 1 subunit

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85916-1-PBS targets ATP1B1 as part of a matched antibody pair:

MP02193-3: 85916-2-PBS capture and 85916-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Eg3007

Product name: Recombinant Mouse Atp1b1 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 63-304 aa of NM_009721.6

Sequence: ELKPTYQDRVAPPGLTQIPQIQKTEISFRPNDPKSYEAYVLNIIRFLEKYKDSAQKDDMIFEDCGNVPSEPKERGDINHERGERKVCRFKLDWLGNCSGLNDDSYGYREGKPCIIIKLNRVLGFKPKPPKNESLETYPLMMKYNPNVLPVQCTGKRDEDKDKVGNIEYFGMGGYYGFPLQYYPYYGKLLQPKYLQPLLAVQFTNLTVDTEIRVECKAYGENIGYSEKDRFQGRFDVKIEIKS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATPase, Na+/K+ transporting, beta 1 polypeptide
别名 ATP1B 1, Atp4b, Beta 1 subunit of Na(+) K(+) ATPase, Na, K-ATPase β1, Na+/K+ ATPase beta 1 subunit
计算分子量35 kDa
观测分子量 40-50 kDa
GenBank蛋白编号NM_009721.6
基因名称 Atp1b1
Gene ID (NCBI) 11931
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP14094
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATP1B1 is one of beta subunits of the Na+/K+ ATPase and responsible for formation and structural integrity of the Na+/K+ ATPase. The Na+/K+ ATPase is a plasma membrane pump consisting of alpha, beta, and gamma subunits. At least four of Na+/K+-ATPase beta subunits (β1, β2, β3, β4) have been identified in mammalian cells; the β1-subunit (ATP1B1) is the most ubiquitous. The Na+/K+ ATPase β subunits have multiple N-glycosylation sites. The predicted MW of ATP1B1 is 35 kDa, while it migrates around 40-52 kDa due to the variable glycosylation. (PMID: 10896885, 17714085)