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验证数据展示

  • WB analysis using 85916-1-RR (same clone as 85916-1-PBS)

    WB analysis using 85916-1-RR (same clone as 85916-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85916-1-RR (Atp1b1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85916-1-PBS in a different storage buffer formulation.

  • WB analysis using 85916-1-RR (same clone as 85916-1-PBS)

    WB analysis using 85916-1-RR (same clone as 85916-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85916-1-RR (Atp1b1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85916-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02193-3

    Sandwich ELISA sample test of MP02193-3

    The mean ATP1B1 concentration was determined to be 58,169.6 pg/mL in mouse heart extract based on a 6.9 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02193-3

    Sandwich ELISA standard curve of MP02193-3

    Sandwich ELISA standard curve of MP02193-3, Mouse ATP1B1 Recombinant Matched Antibody Pair - PBS only. 85916-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3007. 85916-1-PBS was HRP conjugated as the detection antibody. Range: 46.9-3000 pg/mL.

  • IHC staining of mouse liver using 85916-1-RR (same clone as 85916-1-PBS)

    IHC staining of mouse liver using 85916-1-RR (same clone as 85916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85916-1-RR (ATP1B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85916-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 85916-1-RR (same clone as 85916-1-PBS)

    IHC staining of mouse brain using 85916-1-RR (same clone as 85916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85916-1-RR (ATP1B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85916-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 85916-1-RR (same clone as 85916-1-PBS)

    IF Staining of mouse heart using 85916-1-RR (same clone as 85916-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using ATP1B1 antibody (85916-1-RR, Clone: 243093E3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85916-1-PBS in a different storage buffer formulation.

Mouse ATP1B1 Recombinant monoclonal antibody, PBS Only (Detector)

ATP1B1 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Sandwich ELISA, Indirect ELISA
Cat No. 85916-1-PBS

产品说明书

CloneNo. 243093E3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Sandwich ELISA, Indirect ELISA

别名

ATP1B 1, Atp4b, Beta 1 subunit of Na(+) K(+) ATPase, Na, K-ATPase β1, Na+/K+ ATPase beta 1 subunit

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85916-1-PBS targets ATP1B1 as part of a matched antibody pair:

MP02193-3: 85916-2-PBS capture and 85916-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Eg3007

Product name: Recombinant Mouse ATP1B1 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 63-304 aa of NM_009721.6

Sequence: ELKPTYQDRVAPPGLTQIPQIQKTEISFRPNDPKSYEAYVLNIIRFLEKYKDSAQKDDMIFEDCGNVPSEPKERGDINHERGERKVCRFKLDWLGNCSGLNDDSYGYREGKPCIIIKLNRVLGFKPKPPKNESLETYPLMMKYNPNVLPVQCTGKRDEDKDKVGNIEYFGMGGYYGFPLQYYPYYGKLLQPKYLQPLLAVQFTNLTVDTEIRVECKAYGENIGYSEKDRFQGRFDVKIEIKS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATPase, Na+/K+ transporting, beta 1 polypeptide
别名 ATP1B 1, Atp4b, Beta 1 subunit of Na(+) K(+) ATPase, Na, K-ATPase β1, Na+/K+ ATPase beta 1 subunit
计算分子量35 kDa
观测分子量 40-50 kDa
GenBank蛋白编号NM_009721.6
基因名称 Atp1b1
Gene ID (NCBI) 11931
RRIDAB_3744471
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP14094
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATP1B1 is one of beta subunits of the Na+/K+ ATPase and responsible for formation and structural integrity of the Na+/K+ ATPase. The Na+/K+ ATPase is a plasma membrane pump consisting of alpha, beta, and gamma subunits. At least four of Na+/K+-ATPase beta subunits (β1, β2, β3, β4) have been identified in mammalian cells; the β1-subunit (ATP1B1) is the most ubiquitous. The Na+/K+ ATPase β subunits have multiple N-glycosylation sites. The predicted MW of ATP1B1 is 35 kDa, while it migrates around 40-52 kDa due to the variable glycosylation. (PMID: 10896885, 17714085)

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