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验证数据展示

  • WB analysis using 85916-1-RR

    WB analysis using 85916-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85916-1-RR (Atp1b1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 85916-1-RR

    WB analysis using 85916-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85916-1-RR (Atp1b1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 85916-1-RR

    IHC staining of mouse liver using 85916-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85916-1-RR (ATP1B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 85916-1-RR

    IHC staining of mouse brain using 85916-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85916-1-RR (ATP1B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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ATP1B1 Recombinant monoclonal antibody

ATP1B1 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 85916-1-RR

产品说明书

CloneNo. 243093E3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ATP1B 1, Atp4b, Beta 1 subunit of Na(+) K(+) ATPase, Na, K-ATPase β1, Na+/K+ ATPase beta 1 subunit

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, fetal human brain tissue, mouse heart tissue, rat heart tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85916-1-RR targets ATP1B1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Eg3007

Product name: Recombinant Mouse Atp1b1 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 63-304 aa of NM_009721.6

Sequence: ELKPTYQDRVAPPGLTQIPQIQKTEISFRPNDPKSYEAYVLNIIRFLEKYKDSAQKDDMIFEDCGNVPSEPKERGDINHERGERKVCRFKLDWLGNCSGLNDDSYGYREGKPCIIIKLNRVLGFKPKPPKNESLETYPLMMKYNPNVLPVQCTGKRDEDKDKVGNIEYFGMGGYYGFPLQYYPYYGKLLQPKYLQPLLAVQFTNLTVDTEIRVECKAYGENIGYSEKDRFQGRFDVKIEIKS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATPase, Na+/K+ transporting, beta 1 polypeptide
别名 ATP1B 1, Atp4b, Beta 1 subunit of Na(+) K(+) ATPase, Na, K-ATPase β1, Na+/K+ ATPase beta 1 subunit
计算分子量35 kDa
观测分子量 40-50 kDa
GenBank蛋白编号NM_009721.6
基因名称 Atp1b1
Gene ID (NCBI) 11931
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP14094
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP1B1 is one of beta subunits of the Na+/K+ ATPase and responsible for formation and structural integrity of the Na+/K+ ATPase. The Na+/K+ ATPase is a plasma membrane pump consisting of alpha, beta, and gamma subunits. At least four of Na+/K+-ATPase beta subunits (β1, β2, β3, β4) have been identified in mammalian cells; the β1-subunit (ATP1B1) is the most ubiquitous. The Na+/K+ ATPase β subunits have multiple N-glycosylation sites. The predicted MW of ATP1B1 is 35 kDa, while it migrates around 40-52 kDa due to the variable glycosylation. (PMID: 10896885, 17714085)

实验方案

Product Specific Protocols
WB protocol for ATP1B1 antibody 85916-1-RRDownload protocol
IHC protocol for ATP1B1 antibody 85916-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols