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Ataxin 2 Monoclonal antibody

Ataxin 2 Monoclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Mouse / IgM

Reactivity

Human, Mouse, Rat

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E8A2

Cat No : 68316-1-Ig

Print datasheet

Synonyms

ataxin 2, ATX2, ATXN2, FLJ46772, SCA2, TNRC13

验证数据展示

  • WB analysis using 68316-1-Ig

    WB analysis using 68316-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68316-1-Ig (Ataxin 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 68316-1-Ig

    IHC staining of rat brain using 68316-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68316-1-Ig (Ataxin 2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 68316-1-Ig

    IHC staining of rat brain using 68316-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68316-1-Ig (Ataxin 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat brain using 68316-1-Ig

    IF Staining of rat brain using 68316-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Ataxin 2 antibody (68316-1-Ig, Clone: 1E8A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of rat brain using 68316-1-Ig

    IF Staining of rat brain using 68316-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Ataxin 2 antibody (68316-1-Ig, Clone: 1E8A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat brain tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68316-1-Ig targets Ataxin 2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF-P, IHC, WB, ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen Ataxin 2 fusion protein Ag17402 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name ataxin 2
Synonyms ataxin 2, ATX2, ATXN2, FLJ46772, SCA2, TNRC13
Calculated Molecular Weight 1313 aa, 140 kDa
Observed Molecular Weight140-150 kDa
GenBank Accession NumberBC114546
Gene Symbol ATXN2
Gene ID (NCBI) 6311
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
UNIPROT IDQ99700
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATXN2 contains a repeat structure with 22 or 23 triplets coding for glutamine and the (CAG)8CAA(CAG)4CAA(CAG)8 sequence; expansion of this domain to a size ≥34 triplets with a pure CAG sequence primarily causes autosomal dominant SCA2 [PMID:18418684], while ATXN2 expansions with CAA interruptions were observed as the cause of Levo-dopa responsive Parkinson's disease [PMID:10668726]. ATXN2 expansions associated with ALS were reported to be interrupted by at least one CAA triplet [PMID:21537950]

实验方案

Product Specific Protocols
WB protocol for Ataxin 2 antibody 68316-1-IgDownload protocol
IHC protocol for Ataxin 2 antibody 68316-1-IgDownload protocol
IF protocol for Ataxin 2 antibody 68316-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols