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验证数据展示

  • WB analysis using 84808-5-RR

    WB analysis using 84808-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84808-5-RR (Arginase-2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. Mouse liver tissue as a negative control.

  • IHC staining of mouse kidney using 84808-5-RR

    IHC staining of mouse kidney using 84808-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84808-5-RR (Arginase-2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARG2 antibody included

Arginase-2 Recombinant antibody

Arginase-2 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 84808-5-RR

产品说明书

CloneNo. 242362A9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

ARG2, 242362A9, ARG 2, Arginase 2, Arginase II

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inU-251 cells, mouse kidney tissue, K-562 cells, Caco-2 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ARG2 Ready-To-Use IHC Kit. ARG2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84808-5-RR targets Arginase-2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Arginase-2 fusion protein Ag6609 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 arginase, type II
别名 ARG2, 242362A9, ARG 2, Arginase 2, Arginase II
计算分子量 39 kDa
观测分子量 39 kDa
GenBank蛋白编号BC001350
基因名称 ARG2
Gene ID (NCBI) 384
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP78540
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Arginase 2 is composed of 354 amino acid residues, including an NH2-terminal presequence for mitochondrial targeting and import. In the mitochondria, ornithine generated by Arginase 2 will give rise to glutamate via ornithine aminotransferase (OAT). Glutamate participates in several transamination reactions, including forming α-ketoglutarate (αKG) that may enter the TCA cycle and increase cycle intermediates and flux.Arginase 1 is mainly expressed in hepatocytes, and mice with a disruption of Arginase 1 gene die soon after birth. Arginase 2 is poorly expressed in hepatocytes, and most highly expressed in kidney, prostate, and immune cells such as monocyte/ macrophages. (PMID: 25234945,PMID: 27214549)

实验方案

Product Specific Protocols
WB protocol for Arginase-2 antibody 84808-5-RRDownload protocol
IHC protocol for Arginase-2 antibody 84808-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols