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验证数据展示

  • WB analysis using 84808-5-RR (same clone as 84808-5-PBS)

    WB analysis using 84808-5-RR (same clone as 84808-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84808-5-RR (Arginase-2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. Mouse liver tissue as a negative control. This data was developed using the same antibody clone with 84808-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 84808-5-RR (same clone as 84808-5-PBS)

    IHC staining of mouse kidney using 84808-5-RR (same clone as 84808-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84808-5-RR (Arginase-2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84808-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARG2 antibody included
  • Affinity and Kinetic Characterization of 84808-5-RR

    Affinity and Kinetic Characterization of 84808-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84808-5-RR against Human Arginase-2 were performed. The affinity constant is below 1 pM.

Arginase-2 Recombinant monoclonal antibody, PBS Only

Arginase-2 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84808-5-PBS

产品说明书

CloneNo. 242362A9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

ARG2, 242362A9, ARG 2, Arginase 2, Arginase II

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ARG2 Ready-To-Use IHC Kit. ARG2 primary antibody included.

产品信息

84808-5-PBS targets Arginase-2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag6609

Product name: Recombinant human ARG2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-354 aa of BC001350

Sequence: MSLRGSLSRLLQTRVHSILKKSVHSVAVIGAPFSQGQKRKGVEHGPAAIREAGLMKRLSSLGCHLKDFGDLSFTPVPKDDLYNNLIVNPRSVGLANQELAEVVSRAVSDGYSCVTLGGDHSLAIGTISGHARHCPDLCVVWVDAHADINTPLTTSSGNLHGQPVSFLLRELQDKVPQLPGFSWIKPCISSASIVYIGLRDVDPPEHFILKNYDIQYFSMRDIDRLGIQKVMERTFDLLIGKRQRPIHLSFDIDAFDPTLAPATGTPVVGGLTYREGMYIAEEIHNTGLLSALDLVEVNPQLATSEEEAKTTANLAVDVIASSFGQTREGGHIVYDQLPTPSSPDESENQARVRI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 arginase, type II
别名 ARG2, 242362A9, ARG 2, Arginase 2, Arginase II
计算分子量 39 kDa
观测分子量 39 kDa
GenBank蛋白编号BC001350
基因名称 ARG2
Gene ID (NCBI) 384
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP78540
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Arginase 2 is composed of 354 amino acid residues, including an NH2-terminal presequence for mitochondrial targeting and import. In the mitochondria, ornithine generated by Arginase 2 will give rise to glutamate via ornithine aminotransferase (OAT). Glutamate participates in several transamination reactions, including forming α-ketoglutarate (αKG) that may enter the TCA cycle and increase cycle intermediates and flux.Arginase 1 is mainly expressed in hepatocytes, and mice with a disruption of Arginase 1 gene die soon after birth. Arginase 2 is poorly expressed in hepatocytes, and most highly expressed in kidney, prostate, and immune cells such as monocyte/ macrophages. (PMID: 25234945,PMID: 27214549)

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