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CoraLite®594-conjugated Apolipoprotein AI Polyclonal antibody

Apolipoprotein AI Polyclonal Antibody for FC (Intra), IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra), IF-P

Conjugate

CoraLite®594 Fluorescent Dye

Cat No : CL594-14427

Print datasheet

Synonyms

Apo AI, ApoA I, APOA1, apolipoprotein A I, Apolipoprotein A1

验证数据展示

  • IF Staining of mouse liver using CL594-14427

    IF Staining of mouse liver using CL594-14427

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite®594 Apolipoprotein AI antibody (CL594-14427) at dilution of 1:200.

  • FC experiment of HepG2 using CL594-14427

    FC experiment of HepG2 using CL594-14427

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Apolipoprotein AI (CL594-14427) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inmouse liver tissue
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 14427-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-14427 targets Apolipoprotein AI in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra), IF-P
Tested Reactivity human, mouse
Immunogen Apolipoprotein AI fusion protein Ag5793 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name apolipoprotein A-I
Synonyms Apo AI, ApoA I, APOA1, apolipoprotein A I, Apolipoprotein A1
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 26-30 kDa
GenBank Accession NumberBC005380
Gene Symbol APOA1
Gene ID (NCBI) 335
RRIDAB_2919806
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02647
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.

实验方案

Product Specific Protocols
IF protocol for CL594 Apolipoprotein AI antibody CL594-14427Download protocol
FC protocol for CL594 Apolipoprotein AI antibody CL594-14427Download protocol
Standard Protocols
Click here to view our Standard Protocols