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验证数据展示

  • Dot Blot experiment using 85854-1-RR

    Dot Blot experiment using 85854-1-RR

    Dot blot analysis was used to confirm the specificity of 85854-1-RR anti-DNA antibody. samples were spotted onto NC and probed with antibody at 5 µg/ml.The amount of sample (μg) spotted is indicated next to each row.

  • IHC staining of human ovary tumor using 85854-1-RR

    IHC staining of human ovary tumor using 85854-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 85854-1-RR (Anti-DNA antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 85854-1-RR

    IHC staining of human tonsillitis using 85854-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 85854-1-RR (Anti-DNA antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 85854-1-RR

    IHC staining of human placenta using 85854-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85854-1-RR (Anti-DNA antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 85854-1-RR

    IHC staining of human placenta using 85854-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85854-1-RR (Anti-DNA antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HeLa using 85854-1-RR

    FC experiment of HeLa using 85854-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug N/a Anti-DNA Recombinant Antibody (85854-1-RR, Clone:250864A1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

DNA Recombinant monoclonal antibody

DNA Uni-rAb® Recombinant Antibody for IHC, FC (Intra), Dot Blot, ELISA
Cat No. 85854-1-RR

产品说明书

CloneNo. 250864A1

宿主/亚型

Rabbit / IgG

种属反应性

n/a

应用

IHC, FC (Intra), Dot Blot, ELISA

别名

circular DNA, double stranded DNA, double-stranded DNA, linear DNA, single stranded DNA

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman placenta tissue, human ovary tumor tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells
Positive Dot Blot detected inN/A

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.2 ug per 10^6 cells in a 100 µl suspension
DOT BLOTDOT BLOT : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85854-1-RR targets DNA in IHC, FC (Intra), Dot Blot, ELISA applications and shows reactivity with n/a samples.

经测试应用 IHC, FC (Intra), Dot Blot, ELISA Application Description
经测试反应性 n/a
免疫原

unknow

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DNA
别名 circular DNA, double stranded DNA, double-stranded DNA, linear DNA, single stranded DNA
基因名称
Gene ID (NCBI)
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNA antibodies are a type of antibody that can specifically bind to DNA and are mainly used to detect and study DNA-related biological processes. This antibody can recognize single-stranded DNA and double-stranded DNA (including linear DNA and circular DNA).

实验方案

Product Specific Protocols
IHC protocol for DNA antibody 85854-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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