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  • Featured Product
  • KD/KO Validated

Annexin V Monoclonal antibody, PBS Only

Annexin V Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E6A8

Cat No : 66245-1-PBS

Print datasheet

Synonyms

Anchorin CII, Annexin 5, annexin A5, Annexin V, ANX5, ANXA5, Calphobindin I, CBP I, Endonexin II, ENX2, Lipocortin V, PAP I, PP4, Thromboplastin inhibitor, VAC alpha, Vascular anticoagulant alpha

验证数据展示

  • WB analysis using 66245-1-Ig (same clone as 66245-1-PBS)

    WB analysis using 66245-1-Ig (same clone as 66245-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66245-1-Ig (Annexin V antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66245-1-Ig (same clone as 66245-1-PBS)

    WB analysis of HeLa using 66245-1-Ig (same clone as 66245-1-PBS)

    WB result of Annexin V antibody (66245-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin V transfected HeLa cells. This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66245-1-Ig (same clone as 66245-1-PBS)

    IF Staining of HeLa using 66245-1-Ig (same clone as 66245-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin V antibody (66245-1-Ig, Clone: 1E6A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

  • WB analysis using 66245-1-Ig (same clone as 66245-1-PBS)

    WB analysis using 66245-1-Ig (same clone as 66245-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66245-1-Ig (Annexin V antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

  • IHC staining of human malignant melanoma using 66245-1-Ig (same clone as 66245-1-PBS)

    IHC staining of human malignant melanoma using 66245-1-Ig (same clone as 66245-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66245-1-Ig (same clone as 66245-1-PBS)

    IF Staining of HeLa using 66245-1-Ig (same clone as 66245-1-PBS)

    Immunofluorescent analysis of HeLa cells using 66245-1-Ig (Annexin V antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

  • IHC staining of human malignant melanoma using 66245-1-Ig (same clone as 66245-1-PBS)

    IHC staining of human malignant melanoma using 66245-1-Ig (same clone as 66245-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66245-1-Ig (same clone as 66245-1-PBS)

    FC experiment of HeLa using 66245-1-Ig (same clone as 66245-1-PBS)

    1X10^6 HeLa cells were stained with 0.2ug Annexin V antibody (66245-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500. This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

产品信息

66245-1-PBS targets Annexin V in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF, FC, IHC, Indirect ELISA
Tested Reactivity human, mouse
Immunogen Annexin V fusion protein Ag1538 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name annexin A5
Synonyms Anchorin CII, Annexin 5, annexin A5, Annexin V, ANX5, ANXA5, Calphobindin I, CBP I, Endonexin II, ENX2, Lipocortin V, PAP I, PP4, Thromboplastin inhibitor, VAC alpha, Vascular anticoagulant alpha
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC001429
Gene Symbol ANXA5
Gene ID (NCBI) 308
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08758
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.