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Annexin A3 Monoclonal antibody

Annexin A3 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A11E4

Cat No : 66405-1-Ig

Print datasheet

Synonyms

35 alpha calcimedin, Annexin 3, annexin A3, Annexin III, ANX3, ANXA3, Lipocortin III, PAP III

验证数据展示

  • WB analysis using 66405-1-Ig

    WB analysis using 66405-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 66405-1-Ig

    IHC staining of human pancreas using 66405-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66405-1-Ig (Annexin A3 antibody at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with ANXA3 antibody included
  • IHC staining of human pancreas using 66405-1-Ig

    IHC staining of human pancreas using 66405-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66405-1-Ig (Annexin A3 antibody at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with ANXA3 antibody included

经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells, L02 cells, human heart tissue, human spleen tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ANXA3 Ready-To-Use IHC Kit. ANXA3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66405-1-Ig targets Annexin A3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human
Immunogen Annexin A3 fusion protein Ag2381 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name annexin A3
Synonyms 35 alpha calcimedin, Annexin 3, annexin A3, Annexin III, ANX3, ANXA3, Lipocortin III, PAP III
Calculated Molecular Weight 322 aa, 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC000871
Gene Symbol ANXA3
Gene ID (NCBI) 306
RRIDAB_2881779
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12429
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Annexin A3 (Anxa3), also named as lipocortin3, placental anticoagulant protein3 (PAP-III), belongs to annexin family. Annexins are calcium-dependent phospholipids-binding high-abundant intracellular proteins. ANXA3 has been reported to play crucial roles in a diverse range of molecular and cellular processes, including ion channel regulation, anticoagulation, anti-inflammation, vesicle traffick-ing, signal transduction, cell migration, and cell proliferation. It also exhibits important roles in cancer progression, metastasis, and drug resistance.

实验方案

Product Specific Protocols
WB protocol for Annexin A3 antibody 66405-1-IgDownload protocol
IHC protocol for Annexin A3 antibody 66405-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols