验证数据展示
经过测试的应用
| Positive IHC detected in | Human brain cancer（Astrocitoma） Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
98708-2-RR targets Alpha Synuclein in IHC, FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | IHC, FC (Intra) Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg5390 Product name: Recombinant human SNCA protein Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 1-140 aa of BC108275 Sequence: MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYEPEA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | synuclein, alpha (non A4 component of amyloid precursor) |
| 别名 | 1, a-synuclein, SNCA, alpha syn, alpha-syn |
| GenBank蛋白编号 | BC108275 |
| 基因名称 | Alpha Synuclein |
| Gene ID (NCBI) | 6622 |
| RRID | AB_3746289 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P37840 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2 - 8°C. Stable for one year after shipment. |
背景介绍
Alpha Synuclein (α-syn) is a 14-19 kDa phosphoprotein that is primarily localize to the presynaptic terminals of mature neurons, where it is involved in synaptic function and plasticity. α-syn has drawn intense interest ever since the late 1990s, when the first α-synuclein missense mutation was identified as a cause of familial Parkinson's disease (PD). Aggregated and hyper-phosphorylated forms of α-syn protein are the pathological hallmark of Lewy body disease, which includes Parkinson's disease (PD), diffuse Lewy body disease (DLBD), and Lewy body variant of Alzheimer's disease (LBV). This antibody can recognize all the isoforms of α-syn.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for Alpha Synuclein antibody 98708-2-RR | Download protocol |
| IHC protocol for Alpha Synuclein antibody 98708-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
