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验证数据展示

  • WB analysis using CL488-66895

    WB analysis using CL488-66895

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66895 (Alpha Actinin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

CoraLite® Plus 488-conjugated Alpha Actinin Monoclonal antibody

Alpha Actinin Monoclonal Antibody for WB
Cat No. CL488-66895

产品说明书

CloneNo. 1E2E1

宿主/亚型

Mouse / IgG2b

种属反应性

Human, rat, mouse

应用

WB

别名

actinin, alpha 1, ACTN1, Alpha Actinin, Alpha actinin 1, F actin cross linking protein, FLJ40884, FLJ54432, Non muscle alpha actinin 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, RAW 264.7 cells
Planning an IHC experiment? We recommend our IHCeasy Alpha Actinin Ready-To-Use IHC Kit. Alpha Actinin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66895-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66895 targets Alpha Actinin in WB applications and shows reactivity with Human, rat, mouse samples.

经测试应用 WB Application Description
经测试反应性 Human, rat, mouse
免疫原 Alpha Actinin fusion protein Ag28383 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 actinin, alpha 1
别名 actinin, alpha 1, ACTN1, Alpha Actinin, Alpha actinin 1, F actin cross linking protein, FLJ40884, FLJ54432, Non muscle alpha actinin 1
计算分子量 103 kDa
观测分子量 100-105 kDa
GenBank蛋白编号BC015766
基因名称 Alpha Actinin
Gene ID (NCBI) 87
RRIDAB_3084282
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP12814
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 Alpha Actinin antibody CL488-66895Download protocol
Standard Protocols
Click here to view our Standard Protocols